Anmelden

This content is Open Access.

Multicellular Human Alveolar Model Composed of Epithelial Cells and Primary Immune Cells for Hazard Assessment

11.0K Views

09:27 min

May 6th, 2020

May 6th, 2020

10,977 Views

0:04

Introduction

1:11

Peripheral Blood Monocytes (PBM) Isolation

3:07

CD14+ Cell Selection

4:00

PBM Seeding and Differentiation

4:46

Human Alveolar Epithelial Tissue Triple Cell Coculture Model

5:22

MDDC and MDM Seeding

7:03

Coculture Model Transfer to Air-Liquid Interface

7:30

Results: Representative Multicellular Human Alveolar Model Hazard Assessment

8:52

Conclusion

Transkript

Presented here is a protocol for primary human blood monocyte isolation as well as their differentiation into macrophages and dendritic cells and assembly with epithelial cells into a multicellular human lung model. Biological responses of cocultures composed of immune cells differentiated from either freshly isolated or thawed monocytes, upon exposure to proinflammatory stimuli, are compared.

Wir verwenden Cookies, um Ihre Erfahrung auf unserer Website zu verbessern.

Indem Sie unsere Website weiterhin nutzen oder auf „Weiter“ klicken, stimmen Sie zu, unsere Cookies zu akzeptieren.