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JoVE Journal

Genetics

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Quantitative Real-Time PCR Evaluation of microRNA Expressions in Mouse Kidney with Unilateral Ureteral Obstruction

6.1K Views

07:36 min

August 27th, 2020

August 27th, 2020

6,059 Views

0:04

Introduction

0:33

Sham and UUO Surgeries

1:52

Collection of Kidney Samples

2:39

Extraction of Total RNA from the Kidney Samples

5:28

Synthesis of cDNA

6:29

Results: Differentially Expressed miRNAs in the Kidneys of UUO Mice

7:08

Conclusion

Transkript

This method makes it possible to provide microRNA expression in kidneys of mice with a wide range of pathological conditions, such as renal fibrosis. The main advantage of this technique is that it enables microRNA expression profiling with high a

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We describe a method for evaluating the microRNA expression in the kidneys of mice with unilateral ureteral obstruction (UUO) by quantitative reverse-transcription polymerase chain reaction. This protocol is suitable for studying kidney microRNA expression profiles in mice with UUO and in the context of other pathological conditions.

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