Quantitative Real-Time PCR Evaluation of microRNA Expressions in Mouse Kidney with Unilateral Ureteral Obstruction
6.2K Views
•
07:36 min
•
August 27th, 2020
DOI :
August 27th, 2020
•Katsunori Yanai1, Shohei Kaneko1, Hiroki Ishii1, Akinori Aomatsu1,2, Kiyonori Ito1, Keiji Hirai1, Susumu Ookawara1, Kenichi Ishibashi3, Yoshiyuki Morishita1
1Division of Nephrology, First Department of Integrated Medicine, Saitama Medical Center, Jichi Medical University, 2Division of Intensive Care Unit, First Department of Integrated Medicine, Saitama Medical Center, Jichi Medical University, 3Department of Medical Physiology, Meiji Pharmaceutical University
Chapters in this video
0:04
Introduction
0:33
Sham and UUO Surgeries
1:52
Collection of Kidney Samples
2:39
Extraction of Total RNA from the Kidney Samples
5:28
Synthesis of cDNA
6:29
Results: Differentially Expressed miRNAs in the Kidneys of UUO Mice
7:08
Conclusion
Related Videos
11:19
Merging Absolute and Relative Quantitative PCR Data to Quantify STAT3 Splice Variant Transcripts
14.8K Views
06:48
Quantitative Real-Time Polymerase Chain Reaction Evaluation of MicroRNA Expression in Kidney and Serum of Mice with Age-Dependent Renal Impairment
2.2K Views
14:05
A Murine Model of Irreversible and Reversible Unilateral Ureteric Obstruction
30.3K Views
05:39
Detection of MicroRNA Expression in the Kidneys of Immunoglobulin A Nephropathic Mice
1.8K Views
12:21
MicroRNA In situ Hybridization for Formalin Fixed Kidney Tissues
14.9K Views
07:11
A Quantitative Detection Method for MicroRNAs in the Kidney of an Ischemic Kidney Injury Mouse Model
1.4K Views
11:47
Using 2-Photon Microscopy to Quantify the Effects of Chronic Unilateral Ureteral Obstruction on Glomerular Processes
2.2K Views
An Immature Murine Model of Reversible Unilateral Ureteral Obstruction
89 Views
04:57
Unilateral Ureteral Obstruction Model for Investigating Kidney Interstitial Fibrosis
368 Views
01:27
Unilateral Ureteral Obstruction Murine Model: A Surgical Procedure to Obstruct the Urinary Flow from a Kidney in Mouse Models
2.8K Views
Copyright © 2025 MyJoVE Corporation. All rights reserved