Quantitative Real-Time PCR Evaluation of microRNA Expressions in Mouse Kidney with Unilateral Ureteral Obstruction
6.1K Views
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07:36 min
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August 27th, 2020
DOI :
August 27th, 2020
•Katsunori Yanai1, Shohei Kaneko1, Hiroki Ishii1, Akinori Aomatsu1,2, Kiyonori Ito1, Keiji Hirai1, Susumu Ookawara1, Kenichi Ishibashi3, Yoshiyuki Morishita1
1Division of Nephrology, First Department of Integrated Medicine, Saitama Medical Center, Jichi Medical University, 2Division of Intensive Care Unit, First Department of Integrated Medicine, Saitama Medical Center, Jichi Medical University, 3Department of Medical Physiology, Meiji Pharmaceutical University
Chapters in this video
0:04
Introduction
0:33
Sham and UUO Surgeries
1:52
Collection of Kidney Samples
2:39
Extraction of Total RNA from the Kidney Samples
5:28
Synthesis of cDNA
6:29
Results: Differentially Expressed miRNAs in the Kidneys of UUO Mice
7:08
Conclusion
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