Name: three-dimensional finger motion tracking during needling: a solution for the kinematic analysis of acupuncture manipulation
Uploaded: 2021-10-28T14:00:00
Description: Watch this Scientific Journal Video about Three-Dimensional Finger Motion Tracking during Needling: A Solution for the Kinematic Analysis of Acupuncture Manipulation at JoVE.com

This work was supported by the National Natural Science Foundation of China (Grant Number. 82174506).

This study was approved by the ethics committee of Yueyang Hospital, affiliated with Shanghai University of Traditional Chinese Medicine (reference no. 2021-062), and each participant signed an informed consent form.
1. Experiment preparations
<ol>
	<li>Camera settings:
	<ol>
		<li>Place three tripods in front of the operation table, and connect them with three cameras.</li>
		<li>Set the shooting parameters of the cameras as follows: resolution 1280 x720 pixels, format MP4,...

<ol>
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This study established the measurement method of the kinematic parameters of AM in vivo and obtained the data of motion amplitude, velocity, and operation time of the six important tracking points on the thumb and forefinger along three axes. Meanwhile, based on the 3D calibration frame, a 3D stick view and corresponding animation of the thumb and forefinger during needling were generated. The thumb and forefinger movement of AM can be fully displayed with the synchronous playback of kinematic parameter curve an...

As a kind of the clinical skills of traditional Chinese medicine (TCM) and physical stimulation, acupuncture manipulation (AM) is often regarded as an important factor that affects the therapeutic effect of acupuncture1,2. Many studies have confirmed that different AM or different stimulation parameters (needling velocity, amplitude, frequency, etc.) of the same AM resulted in different therapeutic effects3,4,5,6,7. Therefore, the measurement of relevant kinematic parameters of AM and correlation analysis with the therapeutic effect can provide useful data support and reference for the clinical treatment with acupuncture8,9.
The measurement of kinematic parameters of the AM began in the 1980s10. In the early days, the electrical signal conversion technology based on variable resistance was mainly used to convert the displacement signal of the needle body into a voltage or current signal for displaying and recording the amplitude and frequency data of AM11. Moreover, the famous ATP-II Chinese medicine acupuncture technique tester II (ATP-II) with this technology currently has been used by many traditional Chinese medicine universities of China12. After that, with the continuous development and innovation of sensor technology, different types of sensors were used to collect kinematic parameters of AM. For example, the three axes electromagnetic motion sensor was attached to the needle handle to acquire needling amplitude and velocity13; the bioelectric signal sensor was placed on the dorsal horn of the animal&#39;s spinal cord to record needling frequency14, etc. Although the quantitative research of AM based on the above two types of technologies has completed the acquisition of relevant kinematic parameters during needling, its main disadvantages are the inability to perform the real-time non-invasive measurement and the change of operating feel caused by the modification of the needle body.
In recent years, motion tracking technology was gradually applied to the quantitative research of AM15,16. Because it is based on the frame-by-frame analysis of needling video, the measurement of acupuncture parameters can be acquired during in vivo operation without modifying the needle body. This technology has been used to measure the kinematic parameters such as amplitude, velocity, acceleration, and frequency of four tracking points of thumb and forefinger during needling in a two-dimensional (2D) plane and established the corresponding finger stick figure15. Some studies also measured the angle change range of interphalangeal (IP) joint of thumb and forefinger with similar technology9,17,18. However, the current studies on AM analysis are still mainly limited to the 2D motion plane, and the number of tracking points is relatively small. So far, there is no complete three-dimensional (3D) kinematics measurement and analysis method for AM, and no related data was published.
To solve the above problems, this study will use 3D motion tracking technology to measure the kinematic parameters of the seven tracking points of hand during needling. This protocol aims to provide a complete technical solution for the kinematic analysis on AM, as well as the further study on the dose-effect correlation of acupuncture.

<table border="1" fo:keep-together.within-page="1" fo:keep-with-next.within-page="always">
	<tbody>
		<tr>
			<td>3D calibration frame</td>
			<td>Any brand</td>
			<td></td>
			<td>15 x 15 x 15 cm</td>
		</tr>
		<tr>
			<td>Acupuncture needles</td>
			<td>Suzhou Medical Appliance Factory</td>
			<td></td>
			<td>0.35 x 40 mm</td>
		</tr>
		<tr>
			<td>Double-sided tape</td>
			<td>Any brand</td>
			<td></td>
			<td>Round, 1 cm-diameter</td>
		</tr>
		<tr>
			<td>Reflective balls</td>
			<td>Simi Reality Motion Systems GmbH</td>
			<td></td>
			<td>6.5 mm-diameter</td>
		</tr>
		<tr>
			<td>SD card</td>
			<td>Western Digital Corporation</td>
			<td></td>
			<td>SDXC UHS-I</td>
		</tr>
		<tr>
			<td>SD card reader</td>
			<td>UGREEN Group Limited</td>
			<td></td>
			<td>USB 3.0</td>
		</tr>
		<tr>
			<td>Simi Motion</td>
			<td>Simi Reality Motion Systems GmbH</td>
			<td></td>
			<td>Ver.8.5.15</td>
		</tr>
		<tr>
			<td>Swab</td>
			<td>Any brand</td>
			<td></td>
			<td>The volume fraction of ethanol is 70%-80%</td>
		</tr>
		<tr>
			<td>Three cameras</td>
			<td>Victor Company of Japan, Limited</td>
			<td></td>
			<td>JVC GC-PX100BAC</td>
		</tr>
		<tr>
			<td>Three tripods</td>
			<td>Any brand</td>
			<td></td>
			<td></td>
		</tr>
	</tbody>
</table>

three-dimensional finger motion tracking during needling: a solution for the kinematic analysis of acupuncture manipulation

Three-dimensional (3D) motion tracking has been used in many fields, such as the researches of sport and medical skills. This experiment aimed to use 3D motion tracking technology to measure the kinematic parameters of the joints of fingers during acupuncture manipulation (AM) and establish three technical indicators "amplitude, velocity and time". This method can reflect the operation characteristics of AM and provide quantitative parameters along three axes of multiple finger joints. The current evidence shows that the method has great potential for future applications such as the study of the dose-effect relationship of acupuncture, teaching, and learning of AM, and the measurement and preservation of famous acupuncturists' AM.

After establishing this experimental method, the lifting-thrusting and twirling skills of basic AM of nineteen acupuncture teachers from the School of Acupuncture-Moxibustion and Tuina of Shanghai University of TCM were measured using 3D motion tracking. According to the definition of a joint coordinate system (JCS) for the shoulder, elbow, wrist, and hand proposed by the Standardization and Terminology Committee (STC) of the International Society of Biomechanics21, seven finger tracking points ha...

Watch this Scientific Journal Video about Three-Dimensional Finger Motion Tracking during Needling: A Solution for the Kinematic Analysis of Acupuncture Manipulation at JoVE.com

Three-Dimensional Finger Motion Tracking during Needling: A Solution for the Kinematic Analysis of Acupuncture Manipulation

This experimental method describes a solution for the kinematic analysis of acupuncture manipulation with three-dimensional finger motion tracking technology.

Three-dimensional (3D) motion tracking has been used in many fields, such as the researches of sport and medical skills. This experiment aimed to use 3D ...

Acupuncture manipulation is well-known as an important basis for acupuncture treatment. The quantitative analysis of acupuncture manipulation can provide data support for its clinical application. The establishment of this movement method is based on three-dimensional motion tracking technology.It opens up a new way for the quantitative research of acupuncture manipulation. This experimental method provides a solution for the stimulation amount of determination of manual acupuncture and the quantitative evaluation for the teaching and the learning of acupuncture manipulation. To begin, take seven reflective balls with a diameter of 6.5 millimeters for attachment on the participant's holding needle hand.Attach one ball on the midpoint of the ulna and radial styloid defined as tracking point wrist joint and one ball at the metacarpophalangeal joint defined as tracking point thumb base joint, one ball at the interphalangeal joint defined as tracking point thumb end joint, attach one ball on the center of thumbnail defined as tracking point thumb tip. Next, attach one ball at the metacarpophalangeal joint defined as tracking point forefinger base joint. Attach one ball at the proximal interphalangeal joint defined as tracking point forefinger middle joint, and one ball on the center of the fore fingernail defined as tracking point forefinger tip.For 3D calibration, place a small 3D calibration frame with eight points on the operating table. Remove move the frame from the table after taking a video of the calibration frame for at least eight seconds. Instruct the participant to perform acupuncture manipulation on the acupuncture joint LI11 of the volunteer, including lifting-thrusting and twirling skills to control the needle to move up and down and rotate with thumb and forefinger.Next, export the videos from the cameras to the designated disk of the computer and rename the 3D calibration videos in camera one as ca-1.mp4. And similarly, rename the videos of cameras two and three. Synchronize all manipulation videos in the video editing software and export them named as lifting-thrusting-1.avi, lifting-thrusting-2. avi, lifting-thrusting-3. avi, twirling-1.avid, twirling-2. avi, and twirling-3. avi respectively.Open the motion capture an analysis software and choose create a new project. Set the project name and project label and click create and save to save the project in the designated disk. Choose the specification followed by points, then select right hand and drag the tracking points from the predefined points box into the used points box.Click on the close button to continue. Next, select specification followed by connections, then click on the new connection and input connection name as forefinger III right. Select the forefinger middle joint right as the starting point and forefinger tip right as the line two point in the same window.Click on the apply and close buttons to finish the establishment of the connection. For adding the new camera groups, right-click on the cameras and select add camera group. Then again, right-click on the cameras, select rename to rename the camera groups as lifting-thrusting camera group and twirling camera group respectively.Right-click on the lifting-thrusting camera group, select add camera and in the tracking box, click on the select file button. Then click on the open existing file, select the operation video lifting-thrusting-1. avi in the next window and click apply to finish the video import.Likewise, import the corresponding calibration video ca-1. mp4 by clicking on the select file in the 3D calibration box and continue importing other operational and corresponding calibration videos. Next, import the twirling skill and calibration videos into the twirling camera group similar to the video import in the lifting-thrusting camera group.For 3D calibration for each camera, expand the lifting-thrusting camera group, right-click on the lifting-thrusting 1 and select properties. In the 3D calibration box, click on the 3D calibration button, then input the description and add eight points by clicking the add point button eight times. Set the name and corresponding XYZ value for each point according to the calibration parameters and click apply.After configuring all points, click each endpoint of the calibration video to finish the 3D calibration and similarly complete the 3D calibration of the other cameras in the same group and the cameras in the twirling camera group. For 3D finger motion tracking, right-click on the lifting-thrusting camera group, select 3D tracking, select all the cameras, and click OK to open the 3D tracking window. Set the track using pattern matching all points for all the cameras and manually click on all the tracking points in the first frame.Click on the search automatically button to start automatic 3D tracking frame by frame. And similarly, complete the motion tracking of the twirling camera group. To export the data, right-click on the lifting-thrusting camera group, select new 3D calculation, select all the cameras, check update data continuously and store data explicitly in file and create 3D data window, then click OK to continue.Next, right-click on the folder lifting-thrusting camera group 3D coordinates, select export to open the export window, and check column headings, tracking names, start time and frequency, time information in the first column X, Y, Z, VX, VY, VZ parameters. Click the export button to export the data file with the customized name and export the twirling camera group data file in the same way. In the present study during lifting-thrusting and twirling skills, the typical coordinate time curves along three axes of each point were recorded.The preliminary analysis of the experimental data showed that the movement, amplitude, and velocity parameters of the metacarpophalangeal joints were the smallest, larger for the interphalangeal joints, and largest for the proximal interphalangeal joints. Because of the minimal movement, the amplitude along the main motion axis during different skills of the wrist joint could be fixed and the movement seemed to occur from the thumb and index finger. Upon comparison of the data derived from ATP2 and the data exported by the motion capture and analysis software, it was found that the shape of the coordinate time curve of TT along the z-axis was similar to the voltage time curve generated by ATP2 during the lifting-thrusting skill.Meanwhile, during twirling skill, the shape of the amplitude time curve along the y-axis of TT was also similar to the voltage time curve of ATP2 and the average operating cycles of these two types of curves were the same. During motion tracking, all the tracking points should be identified correctly to obtain high-precision data. Pattern matching algorithm is recommended for automatic 3D tracking to located points rapidly.

Research

JoVE Journal

Medicine

Heterotypic Three-dimensional In Vitro Modeling of Stromal-Epithelial Interactions During Ovarian Cancer Initiation and Progression

Heterotypic Three-dimensional In Vitro Modeling of Stromal-Epithelial Interactions During Ovarian Cancer Initiation and Progression

Epithelial ovarian cancers (EOCs) are the leading cause of death from gynecological malignancy in Western societies. Despite advances in surgical ...

Human Neuroendocrine Tumor Cell Lines as a Three-Dimensional Model for the Study of Human Neuroendocrine Tumor Therapy

Neuroendocrine tumors (NETs) are rare tumors, with an incidence of two per 100,&#x200A;000 individuals per year, and they account for 0.5% of all human ...

Three-dimensional Co-culture Model for Tumor-stromal Interaction

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Three-Dimensional (3D) Tumor Spheroid Invasion Assay

Three-Dimensional 3D Tumor Spheroid Invasion Assay

Invasion of surrounding normal tissues is generally considered to be a key hallmark of malignant (as opposed to benign) tumors. For some cancers in ...

Adapted Resistance Training Improves Strength in Eight Weeks in Individuals with Multiple Sclerosis

Hip weakness is a common symptom affecting walking ability in people with multiple sclerosis (MS). It is known that resistance strength training (RST) can ...

Three-Dimensional Printing of a Complex Aortic Anomaly

Complex congenital aortic anomalies include diverse types of malformations that may be clinically asymptomatic or present with respiratory or esophageal ...

Whole-Kidney Three-Dimensional Staining with CUBIC

Although conventional pathology provided numerous information about kidney microstructure, it was difficult to know the precise structure of blood ...

FSN Therapy for Knee Osteoarthritis Pain Management

Fu's Subcutaneous Needling for Knee Osteoarthritis Pain

Fu's subcutaneous needling (FSN) is a new acupuncture and dry needling technique based on traditional Chinese medicine. It rapidly produces long-lasting ...

Computer-Aided Three-Dimensional Visualization in the Treatment of Locally Advanced Thyroid Cancer

The diagnosis and treatment of locally advanced thyroid carcinoma are challenging. The challenge lies in the evaluation of the tumor scope and the ...

Improving FSN Therapy in the Rat Model&#58; Minimizing Discomfort and Maximizing Efficiency

Efficacy of Fu's Subcutaneous Needling on Sciatic Nerve Pain: Behavioral and Electrophysiological Changes in a Chronic Constriction Injury Rat Model

Author Spotlight: Unveiling the Therapeutic Effects of FSN Treatment &#8211; Bridging Research and Clinical Applications in Neuropathic Pain

Fu&#39;s subcutaneous needling (FSN), an invented acupuncture technique from traditional Chinese medicine, is used worldwide for pain relief. However, the ...