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JoVE Journal

Medicine

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Whole-Kidney Three-Dimensional Staining with CUBIC

3.3K Views

04:31 min

July 18th, 2022

July 18th, 2022

3,343 Views

0:05

Introduction

0:37

Kidney Fixation, Decolorization, and De-Lipidation

1:45

Whole-Mount Immunofluorescent Staining and Refractive Index (RI) Matching

3:03

Results: Whole-Mount Immunofluorescent Staining for Three-Dimensional (3D) Kidney Imaging

4:00

Conclusion

Transkript

This protocol allows a visualization of three-dimensional structures in full kidney, which can lead to innovative discoveries in kidney research by providing a macroscopic perspective. Whole-mount organ staining is challenging because of the diffi

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The present protocol describes a tissue clearing method and whole-mount immunofluorescent staining for three-dimensional (3D) kidney imaging. This technique can offer macroscopic perspectives in kidney pathology, leading to new biological discoveries.

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