Isolation of Infrapatellar Fat Pad Mesenchymal Stem Cells (IFP-MSCs) from the Femorotibial Joint of Goat Knee
5:16
Maintenance and Expansion of Isolated Cells
7:13
Differentiation Potential of IFP-MSCs
8:49
Results: Expansion, Clonogenic Ability, and Differentiation Potential of IFP-MSCs
11:07
Conclusion
Transkript
Infrapatellar Fat Pad-derived Mesenchymal Stem Cells, or in short, IFP-MSCs, is a relatively less studied stem cell type. This protocol is a simple, reliable, and reproducible method demonstrating the isolation of IFP-MSCs from goat stifle joint.
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Infrapatellar fat pad mesenchymal stem cells (IFP-MSCs) can be isolated easily from the infrapatellar fat pad of the knee joint. They proliferate well in vitro, form CFU-F colonies, and differentiate into adipogenic, chondrogenic, and osteogenic lineages. Herein, the methodology for the isolation, expansion, and differentiation of IFP-MSCs from goat stifle joint is provided.