Purification and Visualization of Influenza A Viral Ribonucleoprotein Complexes

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Introduction

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Disruption of the Influenza A Virion

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Glycerol Gradient Sediment Velocity Centrifugation

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Analysis of the Glycerol Gradient Fractions by SDS Polyacrylamide Gel Electrophoresis

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Concentration of the vRNP Fractions

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Negative Staining of vRNPs

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Representative Results/ Outcome

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Conclusion