Launch the offline analysis software to view the fully synchronized datasets and open the experiment session file by selecting it from the library. Display the drift corrected images by activating the DC tab below the image viewport before selecting the desired data overlays by checking their respective overlay data boxes in the image metadata tab. Other metadata may be plotted as the user desires.
Highlight or scroll through these graphical plots to update the image displayed in the viewport. Access various tools through the image analysis toolbox and data view tabs. Access the FFT for each image through the image analysis to plot live FFT to update it while scrolling through images.
Use the fading of the FFT peaks to determine the time point at which the zeolite nanoparticle structure loses crystallinity. Note the dose conditions in the software using the tag function. Highlight the tag icon and enter the desired tags to denote a specific series of images within the timeline.
Images will be tagged with this text until the tag icon is deselected. To easily filter large data datasets into smaller shareable ones without losing their associated metadata, open the filter panel and adjust the sliders to select only a dose rate equal to or above 500 square angstroms per second. Save the new collection using the name 500 dose rate and above.
Select the images by highlighting them in the timeline or using the filter options. Then export the metadata as a CSV file and the image series as a movie file using the same publish option. The zeolite nanoparticle ZSM5 imaged to determine the threshold dose showed that the dose rate read by the MVS software under high dose rate conditions was 519 electrons per square angstrom per second.
Nanoparticles in the field of view were imaged continuously until the FFT peaks disappeared, indicating crystalline structure degradation. The FFT peaks began disappearing after 42 seconds of continuous imaging. The FFT peaks had completely disappeared at one minute and 20 seconds and a cumulative dose of approximately 60, 000 electrons per square angstrom.
The same process was repeated with a different microscope. The FFT spots fully disappeared after one minute and 50 seconds of continuous imaging and a cumulative dose of 58, 230 electrons per square angstrom.
