1. Animal Ethics
Before performing the long duration hyperthermia on mice make sure that the procedure has been approved by Institutional Animal Care and Use Committee. All the procedures discussed in this paper and shown in the accompanying video were approved by the Institutional Animal Ethics Committee of the National Institute of Immunology.
2. Understanding Hyperthermia
Whole Body Hyperthermia (WBH) is delivered by placing the animals in...

<ol>
	<li>Braude, A. I., Beck, J., Zalesky, M. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Febrile+response+to+bacterial+pyrogens+in+leukemia.">Febrile response to bacterial pyrogens in leukemia.</a> Blood. 16, 1279 (1960).</li><li>Dovern, E. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Hyperthermic+intraperitoneal+chemotherapy+added+to+the+treatment+of+ovarian+cancer.+A+review+of+achieved+results+and+complications.">Hyperthermic intraperitoneal chemotherapy added to the treatment of ovarian cancer. A review of achieved results and complications.</a> Eur. J. Gynaecol. Oncol. 31, 256 (2010).</li><li>Gaworek, J., Mayer, C. T. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Systemic+whole+body+hyperthermia+in+oncology:+fatal+heat+for+tumor+cells.">Systemic whole body hyperthermia in oncology: fatal heat for tumor cells.</a> Pflege. Z. 56, 15 (2003).</li><li>Hegewisch-Becker, S. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Effects+of+whole+body+hyperthermia+(41.8+degrees+C)+on+the+frequency+of+tumor+cells+in+the+peripheral+blood+of+patients+with+advanced+malignancies.">Effects of whole body hyperthermia (41.8 degrees C) on the frequency of tumor cells in the peripheral blood of patients with advanced malignancies.</a> Clin. Cancer Res. 9, 2079 ( ).</li><li>Jia, D. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Inhibition+of+B16+murine+melanoma+metastasis+and+enhancement+of+immunity+by+fever-range+whole+body+hyperthermia.">Inhibition of B16 murine melanoma metastasis and enhancement of immunity by fever-range whole body hyperthermia.</a> Int. J. Hyperthermia. 27, 275 (2011).</li><li>Maluta, S., Dall'oglio, S., Nadalini, L. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Treatment+for+intermediate+and+high-risk+prostate+cancer:+controversial+issues+and+the+role+of+hyperthermia.">Treatment for intermediate and high-risk prostate cancer: controversial issues and the role of hyperthermia.</a> Int. J. Hyperthermia. 26, 765 (2010).</li><li>Moyer, H. R., Delman, K. A. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=The+role+of+hyperthermia+in+optimizing+tumor+response+to+regional+therapy.">The role of hyperthermia in optimizing tumor response to regional therapy.</a> Int. J. Hyperthermia. 24, 251 (2008).</li><li>Muthana, M., Multhoff, G., Pockley, A. G. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Tumour+infiltrating+host+cells+and+their+significance+for+hyperthermia.">Tumour infiltrating host cells and their significance for hyperthermia.</a> Int. J. Hyperthermia. 26, 247 (2010).</li><li>Ostberg, J. R., Repasky, E. A. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Comparison+of+the+effects+of+two+different+whole+body+hyperthermia+protocols+on+the+distribution+of+murine+leukocyte+populations.">Comparison of the effects of two different whole body hyperthermia protocols on the distribution of murine leukocyte populations.</a> Int. J. Hyperthermia. 16, 29 (2000).</li><li>Pritchard, M. T. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Protocols+for+simulating+the+thermal+component+of+fever:+preclinical+and+clinical+experience.">Protocols for simulating the thermal component of fever: preclinical and clinical experience.</a> Methods. 32, 54 (2004).</li><li>Repasky, E. A. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Characterization+of+mild+whole-body+hyperthermia+protocols+using+human+breast,+ovarian,+and+colon+tumors+grown+in+severe+combined+immunodeficient+mice.">Characterization of mild whole-body hyperthermia protocols using human breast, ovarian, and colon tumors grown in severe combined immunodeficient mice.</a> Infect. Dis. Obstet. Gynecol. 7, 19 (1999).</li><li>Willnow, U. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Treatment+of+otherwise+incurable+tumor+diseases+in+childhood+using+whole-body+hyperthermia+and+chemotherapy.">Treatment of otherwise incurable tumor diseases in childhood using whole-body hyperthermia and chemotherapy.</a> Dtsch. Med. Wochenschr. 114, 208 (1989).</li></ol>

We have nothing to disclose.

As far back as 5,000 B.C., Egyptian doctors treated tumors with heat. The Greeks recognized the value of heat in some medical treatments. The most ancient texts of the Law of Moses mention hot springs (Genesis 36:24) to therapeutically elevate body temperature.
The earlier techniques employed either the use of pyrogenic microbial extracts for induction of fever or hot water baths for induction of hyperthermia 1. However none of these approaches could be easily monitored or controlle...

<table border="1">
 <tr>
 <td></td>
 <td></td>
 <td></td>
 <td></td>
 </tr>
 <tr>
 <td>Thermistor</td>
 <td>RS components</td>
 <td>528-8558</td>
 <td></td>
 </tr>
 <tr>
 <td>CO2 Incubator</td>
 <td>Heraeus</td>
 <td>B5060 EK-CO2</td>
 <td></td>
 </tr>
 <tr>
 <td>Mice cages</td>
 <td>Citizen industries ltd.</td>
 <td></td>
 <td>Type B 365X207X190 cm</td>
 </tr>
 <tr>
 <td>Animal feed</td>
 <td>Provimi India</td>
 <td></td>
 <td></td>
 </tr>
 <tr>
 <td>Water bottle for cage</td>
 <td>Local supplier</td>
 <td></td>
 <td>Glass bottle</td>
 </tr>
 <tr>
 <td>Soldering iron</td>
 <td>Solderon, India</td>
 <td></td>
 <td></td>
 </tr>
 <tr>
 <td>50 ml Centrifuge tubes</td>
 <td>BD Biosciences</td>
 <td>352070</td>
 <td></td>
 </tr>
 <tr>
 <td>Timer</td>
 <td>Local supplier</td>
 <td></td>
 <td></td>
 </tr>
 <tr>
 <td>1 ml Syringe with 26G needle</td>
 <td>BD Biosciences</td>
 <td>309602</td>
 <td></td>
 </tr>
 <tr>
 <td>Saline</td>
 <td>Local supplier</td>
 <td></td>
 <td></td>
 </tr>
</table>

protocol for long duration whole body hyperthermia in mice

Hyperthermia is a general term used to define the increase in core body temperature above normal. It is often used to describe the increased core body temperature that is observed during fever. The use of hyperthermia as an adjuvant has emerged as a promising procedure for tumor regression in the field of cancer biology. For this purpose, the most important requirement is to have reliable and uniform heating protocols. We have developed a protocol for hyperthermia (whole body) in mice. In this protocol, animals are exposed to cycles of hyperthermia for 90 min followed by a rest period of 15 min. During this period mice have easy access to food and water. High body temperature spikes in the mice during first few hyperthermia exposure cycles are prevented by immobilizing the animal. Additionally, normal saline is administered in first few cycles to minimize the effects of dehydration. This protocol can simulate fever like conditions in mice up to 12-24 hr. We have used 8-12 weeks old BALB/Cj female mice to demonstrate the protocol.

Watch this Scientific Journal Video about Protocol for Long Duration Whole Body Hyperthermia in Mice at JoVE.com

Protocol for Long Duration Whole Body Hyperthermia in Mice

This paper describes a protocol for whole body hyperthermia in mice that can stimulate fever like conditions up to 12-24 hr.

Hyperthermia is a general term used to define the increase in core body temperature above normal. It is often used to describe the increased core body ...

protocol-for-long-duration-whole-body-hyperthermia-in-mice

Research

JoVE Journal

Medicine

11.5K vistas.  National Institute of Immunology. En este trabajo se describe un protocolo para la hipertermia de todo el cuerpo en ratones que pueden estimular la fiebre como condiciones hasta 12-24 horas.