1. 동물 윤리 마우스에있는 긴 기간 고열을 수행하기 전에 프로 시저가 기관 동물 케어 및 사용위원회에 의해 승인되었음을 확인합니다. 본 논문에서 논의하고 함께 동영상에 나오는 모든 절차는 면역학의 국립 연구소의 기관 동물 윤리위원회에 의해 승인되었습니다. 2. 이해 고열 온 몸이 고열 (WBH)는 39.5로 유지 보육에 배치됩니다 prewarmed 케이지 ° C.에 동물을 배치하여 배달됩니다 프로토콜의 표준화 동안, 온도 센서는 수술 생쥐에 이식되었으며, 체온이 다른 실험 조건에서 모니터링되었다. 이것은 온수 환경에서 동물을 배치시의 기온이 점차 37로 증가 관찰되었다 ° C까지 42 ° C 100 분 (그림 1)의 기간을. 일내부 몸의 온도가 외부 환경 온도보다 더 높은 총을 맞은으로 온도에서 &quot;스파이크&quot;를 발생하는 전자가 흥미입니다. 몸의 온도가 40 ° C를 초과하면, 동물은 스트레스와 코 주변 으러과 같은 탈수의 징후를 표시됩니다. 40 이상 체온을 증가 ° C는 으러와 돌이킬 수없는 스트레스 또는 열 응력에 의한 동물의 죽음으로 이어질 것입니다. 따라서 고열 기간 동안의 온도 상승을 방지하는 것이 중요합니다. 고온 스파이크의이 현상은 고열에 노출시 동물의 물리적 과다와 연결되어 있습니다. 비슷한 온도 상승도 다른 그룹에 의해 관찰 된 환경이 10보다 높은 온도를 갖는에게 소개하는시 동물의 물리적 과다와 관련된되었습니다. 우리의 프로토콜에서는, 마우스는 고열에 노출시 고정되어 있습니다. 으로그림 2에 도시 된 바와 같이, 동물의 고정은 온도 상승을 방지합니다. 우리는이 프로토콜을 입증 8-12주 이전 BALB / CJ 쥐를 사용했습니다. 프로토콜은 특정 유형의 / 마우스 변형으로 제한되지 않습니다. 3. 실험 준비 3.1 보육 그들은 좋은 온도 제어를했기 때문에이 실험은 일반적인 CO 2 인큐베이터에서 수행 할 수 있습니다. CO 2 가스 공급을 차단하고, CO 2 인큐베이터 물 잡고 트레이를 제거합니다. 인큐베이터의 일부 모델에서는이 CO 2 컨트롤러를 해제 할 수 있습니다. 마우스 케이지가 편리하게 내부에 배치 할 수 있도록하는 보육의 선반을 마련합니다. 39.5 ° C.에서 보육의 온도를 설정 3.2 연습장 일반적으로 고열 실험은 한 번에 4 마우스에 수행됩니다. 두 케이지가 필요합니다;에전자는 인큐베이터 내부에 배치되고 다른 하나는 실온에서 외부 배치됩니다. 침구의 패치 케이지 내부에 비 균일 온도에서 결과 시간이 서부 유럽 표준시 될 때 케이지의 침대는 피할 수 있습니다. 외부 케이지는 음식 펠릿와 물 병이 있어야합니다. 3.3 마우스의 고정 관 고열 마우스의 처음 4 사이클의 과정에서 천공 튜브에 고정되어 있습니다. 이러한 고정 튜브는 모든 신체 및 튜브 네 가지가 필요합니다 이상 피어싱 3mm 구멍 50 ML &#39;원심 분리기&#39;튜브로 만들어 할 수 있습니다. 50ml 관은 편리하게 팔주보다 오래된 BALB / CJ 쥐를 고정 할 수 있습니다. 적절한 크기의 튜브는 생쥐의 일부 비정상적으로 다른 변형 또는 아주 어린 50 주 이상 오래된 쥐 필요할 수 있습니다. 장소 고정 튜브 및 보육 내부 케이지는 39.5 ° C.에서 유지 있도록 최소한 2 시간 동안 기다려 케이지와mmobilization 튜브는 인큐베이터의 온도를 달성. 4. 고열의 첫 번째 사이클 마우스는 고열에 노출시 탈수가 발생할 수 있습니다. 따라서 실험을 시작하기 전에, 모든 마우스에 0.5 ML 일반 생리 intraperitoneally을 관리 할 수​​ 있습니다. 1 ML의 주사기와 26 G 바늘을 사용합니다. 신속 장소, 각 고정 튜브에서 한 동물을 보육에 배치 새장을 지참하여 보육의 케이지를 다시 배치합니다. 시간을 기록합니다. 이 인큐베이터에서 같은 장소마다의 창구를 유지하는 것이 필수적입니다. 90 분 후, 보육에서 케이지를 꺼내. 고정 튜브에서 동물을 떼고 음식과 물에 쉽게 접근 상온에서 케이지에 배치합니다. 보육에 다시 빈 고정 튜브를 놓습니다. 15 분 후, 고열의 두 번째 사이클은 0을 전달함으로써 시작됩니다.모든 마우스 5 ML 염분 intraperitoneally. 그리고 나서 50 ML 구멍 고정 튜브에 고정 및 보육에 배치됩니다. 5. 후속 고열 사이클 처음 4 사이클의 경우는 고열에 노출시와 첫 두 사이클에 대한 쥐를 고정하는 것이 필수적이며, 생리 주입은 고열 기간을 시작하기 전에 주어집니다. 나머지 뒤에 고열의 이러한 사이클이 완료되고 나면, 쥐 순응하고 고열에 노출시 활동적인이되지 않습니다. 쥐가 쉽게 휴식 시간 동안 음식과 물을 가져가 생리의 intraperitoneal 주사가 필요하지 않을 수 있습니다. 6. 모든 사이클이 완료 후, 마지막 단계는 동물을 검사하는 것입니다 고열주기 동안 죽음도 없습니다. 모든 동물은 처음에 매우 조심스럽게 지켜되어야한다몇 사이클. 신속하게 코 주위에 으러 찾아보세요. 이 현상은 탈수의 서명입니다. 이러한 증상이있는 마우스는 즉시 실온에서 0.5 ML 정상적인 생리 IP와 휴식을 제공해야한다. 고정 마우스의 대부분은 추가 치료가 필요하지 않으며 성공적으로 스트레스에게 고열을 부담합니다. 필요한 사이클을 완료 한 후, 동물은 나약하고 탈진 보일 수 있지만 신속하게 몇 시간 이내에 회복됩니다. <img src="/files/ftp_upload/3801/3801fig1.jpg" alt="그림 1" /> 그림 1. 케이지는 39.5 ° C.에서 보육에 배치 된 마우스의 핵심 체온의 변화 90 분 후 케이지는 인큐베이터에서 제거되었습니다 25에 배치 ° C. 빨간 동그라미가 높은 온도 상승을 나타냅니다. <img src="/files/ftp_upload/3801/3801fig2.jpg" alt="그림 2" /> 그림 2. 핵심 몸 temperatu의 변화고열주기 동안 고정 쥐를 다시. 동물을 immobilizing 후, 높은 온도의 상승은 우리가 39.5 ° C.에 배치되었을 때 관찰되지 않았다

<ol>
	<li>Braude, A. I., Beck, J., Zalesky, M. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Febrile+response+to+bacterial+pyrogens+in+leukemia.">Febrile response to bacterial pyrogens in leukemia.</a> Blood. 16, 1279 (1960).</li><li>Dovern, E. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Hyperthermic+intraperitoneal+chemotherapy+added+to+the+treatment+of+ovarian+cancer.+A+review+of+achieved+results+and+complications.">Hyperthermic intraperitoneal chemotherapy added to the treatment of ovarian cancer. A review of achieved results and complications.</a> Eur. J. Gynaecol. Oncol. 31, 256 (2010).</li><li>Gaworek, J., Mayer, C. T. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Systemic+whole+body+hyperthermia+in+oncology:+fatal+heat+for+tumor+cells.">Systemic whole body hyperthermia in oncology: fatal heat for tumor cells.</a> Pflege. Z. 56, 15 (2003).</li><li>Hegewisch-Becker, S. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Effects+of+whole+body+hyperthermia+(41.8+degrees+C)+on+the+frequency+of+tumor+cells+in+the+peripheral+blood+of+patients+with+advanced+malignancies.">Effects of whole body hyperthermia (41.8 degrees C) on the frequency of tumor cells in the peripheral blood of patients with advanced malignancies.</a> Clin. Cancer Res. 9, 2079 ( ).</li><li>Jia, D. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Inhibition+of+B16+murine+melanoma+metastasis+and+enhancement+of+immunity+by+fever-range+whole+body+hyperthermia.">Inhibition of B16 murine melanoma metastasis and enhancement of immunity by fever-range whole body hyperthermia.</a> Int. J. Hyperthermia. 27, 275 (2011).</li><li>Maluta, S., Dall'oglio, S., Nadalini, L. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Treatment+for+intermediate+and+high-risk+prostate+cancer:+controversial+issues+and+the+role+of+hyperthermia.">Treatment for intermediate and high-risk prostate cancer: controversial issues and the role of hyperthermia.</a> Int. J. Hyperthermia. 26, 765 (2010).</li><li>Moyer, H. R., Delman, K. A. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=The+role+of+hyperthermia+in+optimizing+tumor+response+to+regional+therapy.">The role of hyperthermia in optimizing tumor response to regional therapy.</a> Int. J. Hyperthermia. 24, 251 (2008).</li><li>Muthana, M., Multhoff, G., Pockley, A. G. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Tumour+infiltrating+host+cells+and+their+significance+for+hyperthermia.">Tumour infiltrating host cells and their significance for hyperthermia.</a> Int. J. Hyperthermia. 26, 247 (2010).</li><li>Ostberg, J. R., Repasky, E. A. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Comparison+of+the+effects+of+two+different+whole+body+hyperthermia+protocols+on+the+distribution+of+murine+leukocyte+populations.">Comparison of the effects of two different whole body hyperthermia protocols on the distribution of murine leukocyte populations.</a> Int. J. Hyperthermia. 16, 29 (2000).</li><li>Pritchard, M. T. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Protocols+for+simulating+the+thermal+component+of+fever:+preclinical+and+clinical+experience.">Protocols for simulating the thermal component of fever: preclinical and clinical experience.</a> Methods. 32, 54 (2004).</li><li>Repasky, E. A. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Characterization+of+mild+whole-body+hyperthermia+protocols+using+human+breast,+ovarian,+and+colon+tumors+grown+in+severe+combined+immunodeficient+mice.">Characterization of mild whole-body hyperthermia protocols using human breast, ovarian, and colon tumors grown in severe combined immunodeficient mice.</a> Infect. Dis. Obstet. Gynecol. 7, 19 (1999).</li><li>Willnow, U. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Treatment+of+otherwise+incurable+tumor+diseases+in+childhood+using+whole-body+hyperthermia+and+chemotherapy.">Treatment of otherwise incurable tumor diseases in childhood using whole-body hyperthermia and chemotherapy.</a> Dtsch. Med. Wochenschr. 114, 208 (1989).</li></ol>

우리는 공개 할 아무 것도 없습니다.

<html> 까지 거슬러 5000 BC으로 이집트 의사들은 열을 종양을 치료. 그리스인들은 어떤 의학적 치료에서의 열 값을 인식했습니다. 모세 언급 온천 (창세기 36:24)의 법의 가장 오래된 텍스트는 therapeutically 체온을 상승합니다. 이전 기술은 고열 1 유도를위한 열 또는 온수 욕조의 유도를위한 pyrogenic 미생물 추출물의 사용 중 하나를 고용. 그러나 이러한 접근 방법 중 어느 ?...

<table border="1"><tr><td></td><td></td><td></td><td></td></tr><tr><td> 서미스터 </td><td> RS 구성 요소를 </td><td> 528-8558 </td><td></td></tr><tr><td> CO 2 인큐베이터 </td><td> Heraeus </td><td> B5060 EK-CO2 </td><td></td></tr><tr><td> 마우스 케이지 </td><td> 시민 산업 LTD. </td><td></td><td> 타입 B 365X207X190 cm </td></tr><tr><td> 동물 피드 </td><td> Provimi 인도 </td><td></td><td></td></tr><tr><td> 케이지 물 병 </td><td> 지역 업체 </td><td></td><td> 유리 병 </td></tr><tr><td> 납땜 인두 </td><td> Solderon, 인도 </td><td></td><td></td></tr><tr><td> 50 ML의 원심 분리기 튜브 </td><td> BD Biosciences </td><td> 352070 </td><td></td></tr><tr><td> 타이머 </td><td> 지역 업체 </td><td></td><td></td></tr><tr><td> 26G 바늘 1 ML의 주사기 </td><td> BD Biosciences </td><td> 309602 </td><td></td></tr><tr><td> 함수호 </td><td> 지역 업체 </td><td></td><td></td></tr></table>

protocol for long duration whole body hyperthermia in mice

고열은 정상 위의 핵심 체온의 증가를 정의하는 데 사용하는 일반적인 용어입니다. 그것은 종종 발열 동안 관찰 증가의 핵심 체온을 설명하는 데 사용됩니다. 도움이되는 등 고열의 사용은 암 생물학 분야의 종양 회귀를위한 유망 절차로 부각되었습니다. 이러한 목적을 위해, 가장 중요한 요구 사항은 신뢰성과 균일 한 가열 프로토콜을하는 것입니다. 우리는 쥐 고열 (몸 전체)에 대한 프로토콜을 개발했습니다. 이 프로토콜에서는, 동물은 15 분의 휴식 기간 뒤에 90 분에 고열의 사이클에 노출되어있다. 이 기간 동안 마우스는 음식과 물에 쉽게 액세스 할 수 있습니다. 처음 몇 고열에 노출 사이클 동안 쥐 높은 체온 상승은 동물을 immobilizing에 의해 차단되어 있습니다. 또한, 정상적인 생리는 탈수의 영향을 최소화하기 위해 처음 몇주기에 실시됩니다. 이 프로토콜은 최대 12-24 시간에 쥐 조건과 같은 열 시뮬레이션 할 수 있습니다. 우리는이프로토콜을 설명하기 8-12주 이전 BALB / CJ 여자 마우스를 사용했습니다.

Watch this Scientific Journal Video about Protocol for Long Duration Whole Body Hyperthermia in Mice at JoVE.com

Protocol for Long Duration Whole Body Hyperthermia in Mice

본 논문은 최대 12-24 시간에 조건 같은 열 자극 쥐 온 몸이 고열에 대한 프로토콜을 설명합니다.

마우스의 긴 기간 온 몸이 고열에 대한 프로토콜

Hyperthermia is a general term used to define the increase in core body temperature above normal. It is often used to describe the increased core body ...

protocol-for-long-duration-whole-body-hyperthermia-in-mice

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11.5K 조회수.  National Institute of Immunology. 본 논문은 최대 12-24 시간에 조건 같은 열 자극 쥐 온 몸이 고열에 대한 프로토콜을 설명합니다.

비디오: Protocol for Long Duration Whole Body Hyperthermia in Mice

Parabiosis in Mice: A Detailed Protocol

Parabiosis is a surgical union of two organisms allowing sharing of the blood circulation. Attaching the skin of two animals promotes formation of microvasculature at the site of inflammation. Parabiotic partners share their circulating antigens and thus are free of adverse immune reaction. First described by Paul Bert in 18641, the parabiosis surgery was refined by Bunster and Meyer in 1933 to improve animal survival2. In the current protocol, two mice are surgically joined following a modification of the Bunster and Meyer technique. Animals are connected through the elbow and knee joints followed by attachment of the skin allowing firm support that prevents strain on the sutured skin. Herein, we describe in detail the parabiotic joining of a ubiquitous GFP expressing mouse to a wild type (WT) mouse. Two weeks after the procedure, the pair is separated and GFP positive cells can be detected by flow cytometric analysis in the blood circulation of the WT mouse. The blood chimerism allows one to examine the contribution of the circulating cells from one animal in the other.

Parabiotic joining of two organisms leads to the development of a shared circulatory system. In this protocol, we describe the surgical steps to form a parabiotic connection between a wild-type mouse and a constitutive GFP-expressing mouse.

마우스의 Parabiosis : 자세한 프로토콜

Parabiosis is a surgical union of two organisms allowing sharing of the blood circulation. Attaching the skin of two animals promotes formation of ...

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의학

Carotid Artery Infusions for Pharmacokinetic and Pharmacodynamic Analysis of Taxanes in Mice

When proposing the use of a drug, drug combination, or drug delivery into a novel system, one must assess the pharmacokinetics of the drug in the study model. As the use of mouse models are often a vital step in preclinical drug discovery and drug development1-8, it is necessary to design a system to introduce drugs into mice in a uniform, reproducible manner. Ideally, the system should permit the collection of blood samples at regular intervals over a set time course. The ability to measure drug concentrations by mass-spectrometry, has allowed investigators to follow the changes in plasma drug levels over time in individual mice1, 9, 10. In this study, paclitaxel was introduced into transgenic mice as a continuous arterial infusion over three hours, while blood samples were simultaneously taken by retro-orbital bleeds at set time points. Carotid artery infusions are a potential alternative to jugular vein infusions, when factors such as mammary tumors or other obstructions make jugular infusions impractical. Using this technique, paclitaxel concentrations in plasma and tissue achieved similar levels as compared to jugular infusion. In this tutorial, we will demonstrate how to successfully catheterize the carotid artery by preparing an optimized catheter for the individual mouse model, then show how to insert and secure the catheter into the mouse carotid artery, thread the end of the catheter out through the back of the mouse&#8217;s neck, and hook the mouse to a pump to deliver a controlled rate of drug influx. Multiple low volume retro-orbital bleeds allow for analysis of plasma drug concentrations over time.

This method was developed with the goal of delivering a steady drug solution via the carotid artery, to assess the pharmacokinetics of novel drugs in mouse models.

When proposing the use of a drug, drug combination, or drug delivery into a novel system, one must assess the pharmacokinetics of the drug in the study ...

Long-term Blood Pressure Measurement in Freely Moving Mice Using Telemetry

During the development of new vasoactive agents, arterial blood pressure monitoring is crucial for evaluating the efficacy of the new proposed drugs. Indeed, research focusing on the discovery of new potential therapeutic targets using genetically altered mice requires a reliable, long-term assessment of the systemic arterial pressure variation. Currently, the gold standard for obtaining long-term measurements of blood pressure in ambulatory mice uses implantable radio-transmitters, which require&#160;artery cannulation. This technique eliminates the need for tethering, restraining, or anesthetizing the animals which introduce stress and artifacts during data sampling. However, arterial blood pressure monitoring in mice via catheterization can be rather challenging due to the small size of the arteries. Here we present a step-by-step guide to illustrate the crucial key passages for a successful subcutaneous implantation of radio-transmitters and carotid artery cannulation in mice. We also include examples of long-term blood pressure activity taken from freely moving mice after a period of post-surgery recovery. Following this procedure will allow reliable direct blood pressure recordings from multiple animals simultaneously.

The goal of this protocol is to assess systemic blood pressure in conscious freely moving mice using implantable radio-telemetry devices.

텔레 메 트리를 사용하여 자유롭게 이동 마우스의 장기 혈압 측정

During the development of new vasoactive agents, arterial blood pressure monitoring is crucial for evaluating the efficacy of the new proposed drugs. ...

Body Composition and Metabolic Caging Analysis in High Fat Fed Mice

Alterations to body composition (fat or lean mass), metabolic parameters such as whole-body oxygen consumption, energy expenditure, and substrate utilization, and behaviors such as food intake and physical activity can provide important information regarding the underlying mechanisms of disease. Given the importance of body composition and metabolism to the development of obesity and its subsequent sequelae, it is necessary to make accurate measures of these parameters in the pre-clinical research setting. Advances in technology over the past few decades have made it possible to derive these measures in rodent models in a non-invasive and longitudinal fashion. Consequently, these metabolic measures have proven useful when assessing the response of genetic manipulations (for example knockout or transgenic mice, viral knock-down or overexpression of genes), experimental drug/compound screening and dietary, behavioral or physical activity interventions. Herein, we describe the protocols used to measure body composition and metabolic parameters using an animal monitoring system in chow-fed and high fat diet-fed mice.

This protocol describes the use of a body composition analyzer and metabolic animal monitoring system to characterize body composition and metabolic parameters in mice. An obesity model induced by high-fat feeding is used as an example for the application of these techniques.

신체 구성 및 높은 지방 연 준 쥐에서 대사 배우면 분석

Alterations to body composition (fat or lean mass), metabolic parameters such as whole-body oxygen consumption, energy expenditure, and substrate ...

Protocol to Create Chronic Wounds in Diabetic Mice

Chronic wounds develop as a result of defective regulation in one or more complex cellular and molecular processes involved in proper healing. They impact ~6.5M people and cost ~$40B/year in the US alone. Although a significant effort has been invested in understanding how chronic wounds develop in humans, fundamental questions remain unanswered. Recently, we developed a novel mouse model for diabetic chronic wounds that have many characteristics of human chronic wounds. Using db/db-/- mice, we can generate chronic wounds by inducing high levels of oxidative stress (OS) in the wound tissue immediately after wounding, using a one-time treatment with inhibitors specific to the antioxidant enzymes catalase and glutathione peroxidase. These wounds have high levels of OS, develop biofilm naturally, become fully chronic within 20 days after treatment and can remain open more for more than 60 days. This novel model has many features of diabetic chronic wounds in humans and therefore can contribute significantly to advancing fundamental understanding of how wounds become chronic. This is a major breakthrough because chronic wounds in humans cause significant pain and distress to patients and result in amputation if unresolved. Moreover, these wounds are very expensive and time-consuming to treat, and lead to significant loss of personal income to patients. Advancements in this field of study through the use of our chronic wound model can significantly improve health care for millions who suffer under this debilitating condition. In this protocol, we describe in great detail the procedure to cause acute wounds to become chronic, which has not been done before.

Chronic wounds are developed from acute wounds on a diabetic mouse model by inducing high levels of oxidative stress after a full-thickness cutaneous wound. The wound is treated with inhibitors for catalase and glutathione peroxidase, resulting in impaired healing and biofilm development by bacteria present in the skin microbiome.

당뇨병 마우스에서 만성 상처를 만드는 프로토콜

Chronic wounds develop as a result of defective regulation in one or more complex cellular and molecular processes involved in proper healing. They impact ...

Handheld Metal Detector Screening for Metallic Foreign Body Ingestion in Children

Coins are the most common ingested metallic foreign bodies among children. The goal of this protocol is to assess the accuracy and feasibility of using a handheld metal detector to detect ingested metallic foreign bodies in children. We propose that by introducing handheld metal detector screening early in the triage process of children with high suspicion of metallic foreign body ingestion, the number of radiographs being ordered to localize the metallic foreign body can be reduced in this radio-sensitive population. The study protocol requires the screening of the participants for history of foreign body ingestion and exclusion of patients with respiratory distress or metallic implants. The patient changes to hospital gown and items that could contain metal like eyeglasses, earrings, pendants, and ornaments are removed. The patient is positioned in the center of the room away from other metallic interferences. The working status of the handheld metal detector is first confirmed by eliciting a positive audio-visual signal. Then the screening is done in an erect position with head in extension to expose the neck, from the level of the chin to the level of the hip joint, to cover the anatomical areas from neck to pelvis in a zig-zag manner both anteriorly and posteriorly. A positive audio-visual signal is carefully noted during the scanning for the presence of metallic foreign body. Relevant radiographs are ordered as per the area detected on the metal detector screening. The handheld metal detector was able to precisely identify all the coins among the ingested metallic foreign bodies in our study. The handheld metal detector could not consistently detect non-coin metallic foreign bodies. This protocol demonstrates the accuracy of handheld metal detector in the identification and localization of coins and coin like metallic foreign bodies.

Here, we present a protocol to use the handheld metal detector, to screen for the presence of ingested metallic foreign bodies in children who present to the pediatric emergency medicine department with a history of foreign body ingestion.

아이 들에서 금속 이물질 섭취에 대 한 휴대용 금속 탐지기 검사

Coins are the most common ingested metallic foreign bodies among children. The goal of this protocol is to assess the accuracy and feasibility of using a ...

Whole Body and Regional Quantification of Active Human Brown Adipose Tissue Using 18F-FDG PET/CT

In endothermic animals, brown adipose tissue (BAT) is activated to produce heat for defending body temperature in response to cold. BAT&#39;s ability to expend energy has made it a potential target for novel therapies to ameliorate obesity and associated metabolic disorders in humans. Though this tissue has been well studied in small animals, BAT&#39;s thermogenic capacity in humans remains largely unknown due to the difficulties of measuring its volume, activity, and distribution. Identifying and quantifying active human BAT is commonly performed using 18F-Fluorodeoxyglucose (18F-FDG) positron emission tomography and computed tomography (PET/CT) scans following cold-exposure or pharmacological activation. Here we describe a detailed image-analysis approach to quantify total-body human BAT from 18F-FDG PET/CT scans using an open-source software. We demonstrate the drawing of user-specified regions of interest to identify metabolically active adipose tissue while avoiding common non-BAT tissues, to measure BAT volume and activity, and to further characterize its anatomical distribution. Although this rigorous approach is time-consuming, we believe it will ultimately provide a foundation to develop future automated BAT quantification algorithms.

Whole Body and Regional Quantification of Active Human Brown Adipose Tissue Using 18F-FDG PET/CT

Using free, open-source software, we have developed an analytical approach to quantify total and regional brown adipose tissue (BAT) volume and metabolic activity of BAT using 18F-FDG PET/CT.

In endothermic animals, brown adipose tissue (BAT) is activated to produce heat for defending body temperature in response to cold. BAT&#39;s ability to ...

A Whole Body Dosimetry Protocol for Peptide-Receptor Radionuclide Therapy (PRRT): 2D Planar Image and Hybrid 2D+3D SPECT/CT Image Methods

Peptide-receptor-radionuclide-therapy (PPRT) is a targeted therapy that combines a short-range energy radionuclide with a substrate with high specificity for cancer cell receptors. After injection, the radiotracer is distributed throughout the entire body, with a higher uptake in tissues where targeted receptors are overexpressed. The use of beta/gamma radionuclide emitters enables therapy imaging (beta-emission) and post-therapy imaging (gamma-emission) to be performed at the same time. Post-treatment sequential images permit absorbed dose calculation based on local uptake and wash-in/wash-out kinetics. We implemented a hybrid method that combines information derived from both 2D and 3D images. Serial whole-body images and blood samples are acquired to estimate the absorbed dose to different organs at risk and to lesions disseminated throughout the body. A single 3D-SPECT/CT image, limited to the abdominal region, overcomes projection overlap on planar images of different structures such as the intestines and kidneys. The hybrid 2D+3D-SPECT/CT method combines the effective half-life information derived from 2D planar images with the local uptake distribution derived from 3D images. We implemented this methodology to estimate the absorbed dose for patients undergoing PRRT with 177Lu-PSMA-617. The methodology could, however, be implemented with other beta-gamma radiotracers. To date, 10 patients have been enrolled into the dosimetry study with 177Lu-PSMA-617 combined with drug protectors for kidneys and salivary glands (mannitol and glutamate tablets, respectively). The median ratio between kidney uptake at 24 h evaluated on planar images and 3D-SPECT/CT is 0.45 (range:0.32-1.23). The comparison between hybrid and full 3D approach has been tested on one patient, resulting in a 1.6% underestimation with respect to full 3D (2D: 0.829 mGy/MBq, hybrid: 0.315 mGy/MBq, 3D: 0.320 mGy/MBq). Treatment safety has been confirmed, with a mean absorbed dose of 0.73 mGy/MBq (range:0.26-1.07) for kidneys, 0.56 mGy/MBq (0.33-2.63) for the parotid glands and 0.63 mGy/MBq (0.23-1.20) for submandibular glands, values in accordance with previously published data.

A Whole Body Dosimetry Protocol for Peptide-Receptor Radionuclide Therapy PRRT: 2D Planar Image and Hybrid 2D+3D SPECT/CT Image Methods

This method estimates the absorbed dose of different structures for peptide-receptor-radionuclide-therapy (PRRT) with the possibility of avoiding organ overlap on 2D-projections. Serial whole-body planar images permit estimation of mean absorbed doses along the whole body, while the hybrid approach, combining planar images and 3D-SPECT/CT image, overcomes the limitations of structure overlapping.

펩타이드 수용체 방사성 핵종 치료 (PRRT)에 대한 전신 Dosimetry 프로토콜 : 2D 평면 이미지 및 하이브리드 2D + 3D SPECT / CT 이미지 방법

Peptide-receptor-radionuclide-therapy (PPRT) is a targeted therapy that combines a short-range energy radionuclide with a substrate with high specificity ...

Assessing Whole-Body Lipid-Handling Capacity in Mice

Assessing lipid metabolism is a cornerstone of evaluating metabolic function, and it is considered essential for in vivo metabolism studies. Lipids are a class of many different molecules with many pathways involved in their synthesis and metabolism. A starting point for evaluating lipid hemostasis for nutrition and obesity research is needed. This paper describes three easy and accessible methods that require little expertise or practice to master, and that can be adapted by most labs to screen for lipid-metabolism abnormalities in mice. These methods are (1) measuring several fasting serum lipid molecules using commercial kits (2) assaying for dietary lipid-handling capability through an oral intralipid tolerance test, and (3) evaluating the response to a pharmaceutical compound, CL 316,243, in mice. Together, these methods will provide a high-level overview of lipid handling capability in mice.

This paper provides three easy and accessible assays for assessing lipid metabolism in mice.

마우스에서 전신 지질 처리 용량 평가

Assessing lipid metabolism is a cornerstone of evaluating metabolic function, and it is considered essential for in vivo metabolism studies. Lipids are a ...

Short-Duration Hypothermia Induction in Rats using Models for Studies examining Clinical Relevance and Mechanisms

Therapeutic hypothermia (TH) is a powerful neuroprotective strategy that has provided robust evidence for neuroprotection in pre-clinical studies of neurological disorders. Despite strong pre-clinical evidence, TH has not shown efficacy in clinical trials of most neurological disorders. The only successful trials employing therapeutic hypothermia were related to cardiac arrest in adults and hypoxic ischemic injury in neonates. Further investigations into the parameters of its use, and study design comparisons between pre-clinical and clinical studies, are warranted. This article demonstrates two methods of short-duration hypothermia induction. The first method allows for rapid hypothermia induction in rats using ethanol spray and fans. This method works by cooling the skin, which has been less commonly used in clinical trials and may have different physiological effects. Cooling is much more rapid with this technique than is achievable in human patients due to differences in surface area to volume ratio. Along with this, a second method is also presented, which allows for a clinically achievable cooling rate for short-duration hypothermia. This method is easy to implement, reproducible and does not require active skin cooling.

This article describes two methods of whole-body short-duration hypothermia induction in rats. The first, rapid induction method, employs active cooling using fans and ethanol spray for a rapid decrease in temperature. The second method is a gradual cooling method. This is achieved using the combination of isoflurane anesthesia and the reduction of temperature settings on the homeothermic heat mat. This results in a gradual decrease in core body temperature without the use of any external cooling devices.&#160;

임상 관련성 및 메커니즘을 검사 하는 연구에 대 한 모델을 사용 하 여 쥐에 짧은 기간 저온증 유도

Therapeutic hypothermia (TH) is a powerful neuroprotective strategy that has provided robust evidence for neuroprotection in pre-clinical studies of ...