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Identification of Post-translational Modifications of Plant Protein Complexes

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10:07 min

February 22nd, 2014

February 22nd, 2014

23,555 Views

0:05

Title

1:37

N. benthamiana and A. thaliana Protein Samples

3:24

Protein Immunoprecipitation

5:32

Protein Digestion

7:48

Results: Identification of Post-translational Modifications of Tagged Proteins

9:13

Conclusion

We describe here a protocol for the purification and characterization of plant protein complexes. We demonstrate that by immunoprecipitating a single protein within a complex, so we can identify its post-translational modifications and its interacting partners.

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