This work is funded by NIH RO1 NS 40433 (K.J.J.).

All procedures executed are approved by the Indiana University School of Medicine Institutional Animal Care and Use Committee and follow National Institute of Health guidelines.
1. Surgical Technique
<ol>
	<li>Maintain aseptic technique during this procedure by using sterile gloves, instruments, and a sterile surgical field according to NIH guidelines25. Sterilize tools before beginning surgery by autoclaving them (see Table of Specific Reagents/Equipment for comp...

<ol>
	<li>Kaufman, M., Bard, J. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=.">.</a> The Anatomical Basis of Mouse Development. , (1999).</li><li>Ashwell, K. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=The+adult+mouse+facial+nerve+nucleus:+morphology+and+musculotopic+organization.">The adult mouse facial nerve nucleus: morphology and musculotopic organization.</a> Journal of Anatomy. 135, 531-538 (1982).</li><li>Sunderland, S. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=A+classification+of+peripheral+nerve+injuries+producing+loss+of+function.">A classification of peripheral nerve injuries producing loss of function.</a> Brain : A Journal Of Neurology. 74, 491-516 (1951).</li><li>Beahrs, T., Tanzer, L., Sanders, V. M., Jones, K. J. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Functional+recovery+and+facial+motoneuron+survival+are+influenced+by+immunodeficiency+in+crush-axotomized+mice.">Functional recovery and facial motoneuron survival are influenced by immunodeficiency in crush-axotomized mice.</a> Experimental Neurology. 221, 225-230 (2010).</li><li>Mesnard, N. A., Haulcomb, M. M., Tanzer, L., Sanders, V., Jones, K. J. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Delayed+functional+recovery+in+presymptomatic+mSOD1G93A+mice+following+facial+nerve+crush+axotomy.">Delayed functional recovery in presymptomatic mSOD1G93A mice following facial nerve crush axotomy.</a> Journal of Neurodegeneration &amp; Regeneration. 4, 21-25 (2013).</li><li>Komiyama, M., Shibata, H., Suzuki, T. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Somatotopic+representation+of+facial+muscles+within+the+facial+nucleus+of+the+mouse.+A+study+using+the+retrograde+horseradish+peroxidase+and+cell+degeneration+techniques.">Somatotopic representation of facial muscles within the facial nucleus of the mouse. A study using the retrograde horseradish peroxidase and cell degeneration techniques.</a> Brain Behav Evol. 24, 144-151 (1984).</li><li>Canh, M. Y., Serpe, C. J., Sanders, V., Jones, K. J. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=CD4(+)+T+cell-mediated+facial+motoneuron+survival+after+injury:+Distribution+pattern+of+cell+death+and+rescue+throughout+the+extent+of+the+facial+motor+nucleus.">CD4(+) T cell-mediated facial motoneuron survival after injury: Distribution pattern of cell death and rescue throughout the extent of the facial motor nucleus.</a> Journal of Neuroimmunology. 181, 93-99 (2006).</li><li>Isokawa-Akesson, M., Komisaruk, B. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Difference+in+projections+to+the+lateral+and+medial+facial+nucleus:+anatomically+separate+pathways+for+rhythmical+vibrissa+movement+in+rats.">Difference in projections to the lateral and medial facial nucleus: anatomically separate pathways for rhythmical vibrissa movement in rats.</a> Exp Brain Res. 65, 385-398 (1987).</li><li>Streit, W., Kreutzberg, G. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Response+of+endogenous+glial+cells+to+motor+neuron+degeneration+induced+by+toxic+ricin.">Response of endogenous glial cells to motor neuron degeneration induced by toxic ricin.</a> The Journal of Comparative Neurology. 268, 248-263 (1988).</li><li>Serpe, C. J., Tetzlaff, J. E., Coers, S., Sanders, V., Jones, K. J. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Functional+recovery+after+facial+nerve+crush+is+delayed+in+severe+combined+immunodeficient+mice.">Functional recovery after facial nerve crush is delayed in severe combined immunodeficient mice.</a> Brain, Behavior, And Immunity. 16, 808-812 (2002).</li><li>Lal, D., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Electrical+stimulation+facilitates+rat+facial+nerve+recovery+from+a+crush+injury.+Otolaryngology--Head+And+Neck+Surgery.">Electrical stimulation facilitates rat facial nerve recovery from a crush injury. Otolaryngology--Head And Neck Surgery.</a> Official Journal Of American Academy Of Otolaryngology-Head And Neck Surgery. 139, 68-73 (2008).</li><li>Tomov, T., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=An+Example+of+Neural+Plasticity+Evoked+by+Putative+Behavioral+Demand+and+Early+Use+of+Vibrissal+Hairs+after+Facial+Nerve+Transection.">An Example of Neural Plasticity Evoked by Putative Behavioral Demand and Early Use of Vibrissal Hairs after Facial Nerve Transection.</a> Experimental Neurology. 178, 207-218 (2002).</li><li>Skouras, E., Angelov, D. N. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Experimental+studies+on+post-transectional+facial+nerve+regrowth+and+functional+recovery+of+paralyzed+muscles+of+the+face+in+rats+and+mice.">Experimental studies on post-transectional facial nerve regrowth and functional recovery of paralyzed muscles of the face in rats and mice.</a> Anatomy (International Journal of Experimental and Clinical Anatomy). 4, 1-27 (2010).</li><li>Xin, J., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=IL-10+within+the+CNS+is+necessary+for+CD4++T+cells+to+mediate+neuroprotection).">IL-10 within the CNS is necessary for CD4+ T cells to mediate neuroprotection).</a> Brain, Behavior, And Immunity. 25, 820-829 (2011).</li><li>Mesnard, N. A., Sanders, V. M., Jones, K. J. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Differential+gene+expression+in+the+axotomized+facial+motor+nucleus+of+presymptomatic+SOD1+mice.">Differential gene expression in the axotomized facial motor nucleus of presymptomatic SOD1 mice.</a> The Journal of Comparative Neurology. 519, 3488-3506 (2011).</li><li>Mesnard, N. A., Alexander, T. D., Sanders, V. M., Jones, K. J. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Use+of+laser+microdissection+in+the+investigation+of+facial+motoneuron+and+neuropil+molecular+phenotypes+after+peripheral+axotomy.">Use of laser microdissection in the investigation of facial motoneuron and neuropil molecular phenotypes after peripheral axotomy.</a> Experimental Neurology. 225, 94-103 (2010).</li><li>Franchi, G. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Changes+in+motor+representation+related+to+facial+nerve+damage+and+regeneration+in+adult+rats.">Changes in motor representation related to facial nerve damage and regeneration in adult rats.</a> Experimental Brain Research. 135, 53-65 (2000).</li><li>Munera, A., Cuestas, D. M., Troncoso, J. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Peripheral+facial+nerve+lesions+induce+changes+in+the+firing+properties+of+primary+motor+cortex+layer+5+pyramidal+cells.">Peripheral facial nerve lesions induce changes in the firing properties of primary motor cortex layer 5 pyramidal cells.</a> Neuroscience. 223, 140-151 (2012).</li><li>Liu, L., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Hereditary+absence+of+complement+C5+in+adult+mice+influences+Wallerian+degeneration,+but+not+retrograde+responses,+following+injury+to+peripheral+nerve.">Hereditary absence of complement C5 in adult mice influences Wallerian degeneration, but not retrograde responses, following injury to peripheral nerve.</a> Journal of the Peripheral Nervous System. 4, 123-133 (1999).</li><li>Ferri, C., Moore, F., Bisby, M. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Effects+of+facial+nerve+injury+on+mouse+motoneurons+lacking+the+p75+low-affinity+neurotrophin+receptor.">Effects of facial nerve injury on mouse motoneurons lacking the p75 low-affinity neurotrophin receptor.</a> Journal of Neurobiology. 34, 1-9 (1997).</li><li>Zhou, R. Y., Xu, J., Chi, F. L., Chen, L. H., Li, S. T. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Differences+in+sensitivity+to+rocuronium+among+orbicularis+oris+muscles+innervated+by+normal+or+damaged+facial+nerves+and+gastrocnemius+muscle+innervated+by+somatic+nerve+in+rats:+combined+morphological+and+functional+analyses.">Differences in sensitivity to rocuronium among orbicularis oris muscles innervated by normal or damaged facial nerves and gastrocnemius muscle innervated by somatic nerve in rats: combined morphological and functional analyses.</a> The Laryngoscope. 122, 1831-1837 (2012).</li><li>Jinno, S., Yamada, J. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Using+comparative+anatomy+in+the+axotomy+model+to+identify+distinct+roles+for+microglia+and+astrocytes+in+synaptic+stripping.">Using comparative anatomy in the axotomy model to identify distinct roles for microglia and astrocytes in synaptic stripping.</a> Neuron Glia Biology. 7, 55-66 (2011).</li><li>Jones, K. J., Serpe, C. J., Byram, S. C., Deboy, C. A., Sanders, V. M. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Role+of+the+immune+system+in+the+maintenance+of+mouse+facial+motoneuron+viability+after+nerve+injury.">Role of the immune system in the maintenance of mouse facial motoneuron viability after nerve injury.</a> Brain, Behavior, And Immunity. 19, 12-19 (2005).</li><li>Moran, L. B., Graeber, M. B. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=The+facial+nerve+axotomy+model.">The facial nerve axotomy model.</a> Brain research. Brain research. 44, 154-178 (2004).</li><li>Council, N. R. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=.">.</a> Guide for the Care and Use of Laboratory Animals: Eighth Edition. , (2011).</li><li>Serpe, C. J., Kohm, A. P., Huppenbauer, C. B., Sanders, V., Jones, K. J. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Exacerbation+of+Facial+Motoneuron+Loss+after+facial+nerve+transection+in+severe+combined+immunodeficient+(scid)+mice.">Exacerbation of Facial Motoneuron Loss after facial nerve transection in severe combined immunodeficient (scid) mice.</a> Neuroscience. 19, (1999).</li><li>Mesnard-Hoaglin, N. A., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=SOD1(G93A)+transgenic+mouse+CD4(+)+T+cells+mediate+neuroprotection+after+facial+nerve+axotomy+when+removed+from+a+suppressive+peripheral+microenvironment.">SOD1(G93A) transgenic mouse CD4(+) T cells mediate neuroprotection after facial nerve axotomy when removed from a suppressive peripheral microenvironment.</a> Brain, Behavior, And Immunity. 40, 55-60 (2014).</li><li>Wang, H., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Establishment+and+assessment+of+the+perinatal+mouse+facial+nerve+axotomy+model+via+a+subauricular+incision+approach.">Establishment and assessment of the perinatal mouse facial nerve axotomy model via a subauricular incision approach.</a> Experimental Biology And Medicine. 237, 1249-1255 (2012).</li><li>Sharma, N., Moeller, C. W., Marzo, S. J., Jones, K. J., Foecking, E. M. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Combinatorial+treatments+enhance+recovery+following+facial+nerve+crush.">Combinatorial treatments enhance recovery following facial nerve crush.</a> The Laryngoscope. 120, 1523-1530 (2010).</li><li>Lieberman, D. M., Jan, T. A., Ahmad, S. O., Most, S. P. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Effects+of+corticosteroids+on+functional+recovery+and+neuron+survival+after+facial+nerve+injury+in+mice.">Effects of corticosteroids on functional recovery and neuron survival after facial nerve injury in mice.</a> Archives of Facial Plastic Surgery. 13, 117-124 (2011).</li><li>Serpe, C. J., Coers, S., Sanders, V. M., Jones, K. J. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=CD4++T,+but+not+CD8++or+B,+lymphocytes+mediate+facial+motoneuron+survival+after+facial+nerve+transection.">CD4+ T, but not CD8+ or B, lymphocytes mediate facial motoneuron survival after facial nerve transection.</a> Brain, Behavior, And Immunity. 17, 393-402 (2003).</li><li>Haulcomb, M. M., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Axotomy-induced+target+disconnection+promotes+an+additional+death+mechanism+involved+in+motoneuron+degeneration+in+ALS+transgenic+mice.">Axotomy-induced target disconnection promotes an additional death mechanism involved in motoneuron degeneration in ALS transgenic mice.</a> The Journal of Comparative Neurology. , (2014).</li><li>Bauder, A. R., Ferguson, T. A. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Reproducible+mouse+sciatic+nerve+crush+and+subsequent+assessment+of+regeneration+by+whole+mount+muscle+analysis.">Reproducible mouse sciatic nerve crush and subsequent assessment of regeneration by whole mount muscle analysis.</a> Journal of Visualized Experiments : JoVE. (60), (2012).</li><li>Richner, M., Bjerrum, O. J., Nykjaer, A., Vaegter, C. B. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=The+spared+nerve+injury+(SNI)+model+of+induced+mechanical+allodynia+in+mice.">The spared nerve injury (SNI) model of induced mechanical allodynia in mice.</a> Journal of Visualized Experiments : JoVE. (54), (2011).</li></ol>

The critical step for this protocol is positioning the mouse properly before surgery is begun. If the mouse is not lying flat on its side, the ear is not taped at the correct angle, or the incision is made in an incorrect location, then finding the facial nerve becomes much more difficult. When this technique is mastered, surgeries will take only minutes per mouse. 
Either sutures, glue, or wound clips can be used to close the wound. Wound clips are preferred because of the small size of incis...

Many peripheral nerve injury models exist, but one that stands out for the study of motoneurons is the facial nerve axotomy model. The facial nerve, also known as cranial nerve VII, originates in the pons and innervates the muscles of facial expression1,2. In this surgical protocol, the facial nerve is exposed at its exit from the stylomastoid foramen and either cut or crushed. The severity of nerve injury can be classified following the Sunderland3 classifications, which differentiates the injury based on the intactness of the axons, endoneurium, perineurium, and epineurium, which are connective tissue layers that sequentially wrap around the axon bundles. In the crush injury (axonotmesis), the axons are severed, but the perineurium and epineurium are preserved. Complete functional recovery from facial nerve crush occurs in about 11 days because the intact nerve sheath serves as a conduit within which the axons regrow4,5. On the other hand, in the cut injury (neurotmesis), the axons and all 3 connective tissue layers are severed, and the entire distal nerve must regrow to restore musculature innervation. Surgical reconnection of the epineurium is often performed in human patients with nerve transection injuries, however the recovery outcomes are seldom optimal. Further study is required to understand why the nerve fails to regrow to its target and what therapies can be employed to improve and accelerate the regenerative process.
There are many advantages to studying nerve injury using the facial nerve axotomy model. First, the facial nerve axotomy procedure is quick, easy, and highly reproducible; and the resultant paralysis of the facial muscles does not impact vital functions and is well tolerated by the animal. Because this is a cranial nerve injury model, studying the motoneuron cell bodies is simplified because the motoneurons reside in a relatively homogenous population in the facial motor nucleus in the pons. The population does differ based on the subnuclear pattern within the facial motor nucleus, as there are seven subnuclei each specific to innervating a specific group of muscles, so subnuclear differences in response to axotomy may impact results2,6,7.
A major benefit of the facial nerve injury model is that the unaxotomized side can serve as a paired internal control because the nerve innervation is highly symmetrical and there is no crosstalk between the facial motor nuclei8. Another advantage of using this surgical method is the lack of direct trauma to the CNS or disruption of the blood brain barrier9. Complications such as excessive bleeding and infection are rare with this procedure.
A variety of analyses can be performed to assess the physiologic response to nerve injury. The recovery of the eye blink reflex and whisker activity can be used as a behavioral measure of functional recovery10,11. Video recording of vibrissae activity is currently the most powerful method for detecting recovery of facial nerve innervation12,13. After euthanasia, histological analysis of the brainstem can be performed on the motoneuron cell bodies within the facial motor nucleus. The facial motor nucleus is subdivided into seven subnuclei, each specific to certain facial muscles, allowing for differential examination of responses to injury2,6. Facial motoneurons can be counted to quantify cell survival, or immunohistochemistry can be used to identify biomarkers and specific cell populations14. The facial motor nucleus can be accurately microdissected using laser capture for molecular analysis of the cellular response to nerve injury15,16. Impacts of the facial nerve axotomy can be analyzed in the motor cortex17,18. Also, the nerve can be dissected to study Wallerian degeneration19 or axon regeneration20, and the muscles can be removed to study neuromuscular junctions21. The facial nerve axotomy can also be used to study the accompanying central and peripheral glial cells22, target musculature21, and the immune system response23. Although much has been accomplished in studying the facial nerve axotomy model24, further study of peripheral nerve injury is required because nerve damage is a significant problem for patients and current treatments fail to produce optimal results. This model is a powerful tool for examining the physiologic response to nerve injury and analyzing the effectiveness of nerve regeneration therapies.

<table border="0" cellpadding="0" cellspacing="0" width="980">
	<tbody>
		<tr height="21">
			<td height="21" style="height:21px;width:381px;">Name of Material/ Equipment</td>
			<td style="width:167px;">Company</td>
			<td style="width:176px;">Catalog Number</td>
			<td style="width:256px;">Comments/Description</td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;width:381px;">Stereo Microscope</td>
			<td style="width:167px;">Leica</td>
			<td style="width:176px;">M60</td>
			<td></td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;width:381px;">Labeling tape</td>
			<td style="width:167px;">Fisher Scientific</td>
			<td style="width:176px;">15-952</td>
			<td></td>
		</tr>
		<tr height="42">
			<td height="42" style="height:42px;width:381px;">Vannas-T&#252;bingen Spring Scissors - Straight/Sharp/8.5cm/5mm cutting edge</td>
			<td style="width:167px;">Fine Science Tools</td>
			<td style="width:176px;">15003-08</td>
			<td>Sterilize before use</td>
		</tr>
		<tr height="42">
			<td height="42" style="height:42px;width:381px;">Dumont #5/45 Forceps - Standard tips/Angled 45&#176;/Dumoxel/11cm</td>
			<td style="width:167px;">Fine Science Tools</td>
			<td style="width:176px;">11251-35</td>
			<td>Sterilize before use</td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;width:381px;">Michel Suture Clips - 7.5mm x 1.75 mm</td>
			<td style="width:167px;">Fine Science Tools</td>
			<td style="width:176px;">12040-01</td>
			<td>Described as &#34;wound clip&#34; in protocol, sterilize before use</td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;width:381px;">Hagenbarth Cross Action Wound Clip Applier 5&#34;</td>
			<td style="width:167px;">George Tiemann &#38; Co</td>
			<td style="width:176px;">160-910</td>
			<td>Used to apply wound clip, sterilize before use</td>
		</tr>
		<tr height="42">
			<td height="42" style="height:42px;width:381px;">Michel Suture Clip Applicator &#38; Remover - For 7.5 mm Clips</td>
			<td style="width:167px;">Fine Science Tools</td>
			<td style="width:176px;">12029-12</td>
			<td>Used to remove wound clip</td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;width:381px;">0.9% Sodium Chloride Injection, USP</td>
			<td style="width:167px;">Hospira</td>
			<td style="width:176px;">0409-4888-10</td>
			<td></td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;width:381px;">Betadine, 16 oz, with dispenser</td>
			<td style="width:167px;">Fisher Scientific</td>
			<td style="width:176px;">19-027132</td>
			<td></td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;width:381px;">70% Ethanol</td>
			<td style="width:167px;"></td>
			<td style="width:176px;"></td>
			<td></td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;width:381px;">Glass Bead Sterilizer</td>
			<td style="width:167px;"></td>
			<td style="width:176px;"></td>
			<td></td>
		</tr>
	</tbody>
</table>

facial nerve axotomy in mice: a model to study motoneuron response to injury

The goal of this surgical protocol is to expose the facial nerve, which innervates the facial musculature, at its exit from the stylomastoid foramen and either cut or crush it to induce peripheral nerve injury. Advantages of this surgery are its simplicity, high reproducibility, and the lack of effect on vital functions or mobility from the subsequent facial paralysis, thus resulting in a relatively mild surgical outcome compared to other nerve injury models. A major advantage of using a cranial nerve injury model is that the motoneurons reside in a relatively homogenous population in the facial motor nucleus in the pons, simplifying the study of the motoneuron cell bodies. Because of the symmetrical nature of facial nerve innervation and the lack of crosstalk between the facial motor nuclei, the operation can be performed unilaterally with the unaxotomized side serving as a paired internal control. A variety of analyses can be performed postoperatively to assess the physiologic response, details of which are beyond the scope of this article. For example, recovery of muscle function can serve as a behavioral marker for reinnervation, or the motoneurons can be quantified to measure cell survival. Additionally, the motoneurons can be accurately captured using laser microdissection for molecular analysis. Because the facial nerve axotomy is minimally invasive and well tolerated, it can be utilized on a wide variety of genetically modified mice. Also, this surgery model can be used to analyze the effectiveness of peripheral nerve injury treatments. Facial nerve injury provides a means for investigating not only motoneurons, but also the responses of the central and peripheral glial microenvironment, immune system, and target musculature. The facial nerve injury model is a widely accepted peripheral nerve injury model that serves as a powerful tool for studying nerve injury and regeneration.

After the facial nerve axotomy is performed, motoneuron loss occurs as a result of the injury. Motoneuron survival after injury depends on many variables, such as gender, animal age at time of surgery, and the timepoint at which the motoneuron counts are done, and the Moran and Graeber review24 and Jinno and Yamada review22 both summarize motoneuron survival data. Typically, about 86% of motoneurons survive at 28 days post-axotomy14,15,26. Kinetics of motoneuron loss are described in Serp...

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Facial Nerve Axotomy in Mice: A Model to Study Motoneuron Response to Injury

We present a surgical protocol detailing how to perform a cut or crush axotomy on the facial nerve in the mouse. The facial nerve axotomy can be employed to study the physiological response to nerve injury and test therapeutic techniques.

The goal of this surgical protocol is to expose the facial nerve, which innervates the facial musculature, at its exit from the stylomastoid foramen and ...

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12.7K vistas.  Indiana University School of Medicine. We present a surgical protocol detailing how to perform a cut or crush axotomy on the facial nerve in the mouse. The facial nerve axotomy can be employed to study the physiological response to nerve injury and test therapeutic techniques. 