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Purification and Refolding to Amyloid Fibrils of (His)6-tagged Recombinant Shadoo Protein Expressed as Inclusion Bodies in E. coli

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09:43 min

December 19th, 2015

December 19th, 2015

14,025 Views

0:05

Title

0:43

Shadoo Protein Expression and Purification by Low Pressure Liquid Chromatography

3:07

Ni2+-affinity Chromatography

4:51

Size Exclusion Chromatography and Desalting

6:07

In Vitro Assembly of Shadoo into Amyloid Fibrils at Physiological pH and Acidic pHs

8:01

Results: His6-tagged Shadoo Protein Purification and Refolding to Amyloid Fibrils

9:04

Conclusion

A two-step chromatographic method is described for the purification of recombinant Shadoo protein expressed as inclusion bodies in Escherichia coli, as well as a protocol to fibrillate purified Shadoo into amyloid structures.

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