Generation and Expression of Fusion Proteins and Free Dam Proteins
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Library Preparation: Fragmentation
5:29
Library Preparation: Size Selection
7:26
Results: Detecting Lamin B1 Binding Regions on the Mouse Chromosome One
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Conclusion
Transcribir
The overall goal of this protocol is to detect genomic DNA sequences associated with proteins of interest in animal cells by high throughput sequencing. This technique can help answer key questions in the study of nuclear organization and transcri
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We describe herein an assay by coupling DNA adenine methyltransferase identification (DamID) to high throughput sequencing (DamID-seq). This improved method provides a higher resolution and a wider dynamic range, and allows analyzing DamID-seq data in conjunction with other high throughput sequencing data such as ChIP-seq, RNA-seq, etc.