Characterizing Early Elongation Using 4D (Time Lapse Z-stack) DIC Imaging
3:47
Characterizing Late Elongation Using Flow Cytometry
8:23
Results: 4D-DIC versus Flow Cytometry for Sizing Nematode Larvae
9:32
Conclusion
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The overall goal of this group of experiments is to identify developmental defects in the nematode Caenorhabditis elegans during the early and late phases of embryonic elongation. This method can answer a key question in the developmental genetic
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Here we detail protocols specifically designed to monitor morphogenic defects that occur during early and late phases of embryonic elongation of the nematode Caenorhabditis elegans. Ultimately, these protocols are designed to identify genes that regulate these phases and to characterize their differential requirements along the antero-posterior axis of the embryo.