Culturing Fission Yeast Strains for Mating Experiments
3:31
Preparing Agarose Pads and Mounting Cells for Imaging
5:52
Long-term Monitoring of the Reproductive Process in Fission Yeast
7:20
Conclusion
Transcribir
The overall goal of this method is to monitor the entire sexual reproduction process in fission yeast by light microscopy. This method describes the preparation of fission yeast cells for time-lapse imaging of various stages of the sexual lifecycl
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We provide a reproducible basic method for the long-term microscopy of the fission yeast sexual lifecycle. With minor adjustments described, the presented protocol allows research focus on different steps of the reproductive process.