Feeding Paradigm and Mounting Larvae for Live Imaging
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Food Intake Assay
8:08
Results: High-fat Feeding Fluorescent Analogs Allows for Visualization of Transport and Incorporation of Lipids
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Conclusion
Transcribir
The overall goal of this experimental protocol is to feed larval Zebrafish a lipid rich meal that can be spiked with a fluorescent lipid analog to mount the larvae to visual lipid uptake dynamics and to quantify dietary lipid intake. This method c
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Zebrafish are emerging as a valuable model of dietary lipid processing and metabolic disease. Described are protocols of lipid-rich larval feeds, live imaging of dietary fluorescent lipid analogs, and quantification of food intake. These techniques can be applied to a variety of screening, imaging, and hypothesis driven inquiry techniques.