Cloning Genes of Interest in the Multigene Expression System Vector, pMGX
4:11
Insert Gene 2 into the pMGX Vector Containing Gene 1, pMGX-yfg1
7:08
Results: Multigene Expression in pMGX
9:10
Conclusion
Transcripción
The overall goal of this procedure is to construct multigene synthetic operons under the control of an inducible T7 RNA polymerase for expressing multiple proteins simultaneously. This method can help researchers in synthetic biology and biochemis
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This study describes methods for the T7-mediated co-expression of multiple genes from a single plasmid in Escherichia coli using the pMGX plasmid system.