Dye Perfusion (Rhod-2AM for Cystosolic Ca2+ Measurements)
5:59
Recording Epicardial and Endocardial Signals
8:41
Results: AP and Ca2+ Transients in Endocardium and Epicardium
10:02
Conclusion
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The overall goal of this local field fluorescence microscopy imaging technique is to use molecular probes to measure intracellular calcium levels, or membrane potentials from heart regions difficult to access at the whole heart level. This method
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In the heart, molecular events coordinate the electrical and contractile function of the organ. A set of local field fluorescence microscopy techniques presented here enables the recording of cellular variables in intact hearts. Identifying mechanisms defining the cardiac function is critical in understanding how the heart works under pathological situations.