Thymidine- and Nocodazole-based (Thy-noc) Synchronization and Release
3:34
HU-based Synchronization and Release from G1/S Boundary
5:14
Collecting Samples for FACS Analysis and RNA Extraction
7:00
RNA Extraction for Gene Expression Analysis
9:02
Results: Thy-noc and HU Cell Synchronization Protocols Enable Analysis of Gene Expression During Specific Phases of the Cell Cycle
11:07
Conclusion
Transcripción
The overall goal of this protocol is to provide a context for the analysis of gene expression in a cell-cycle specific manner. This method can help answer key questions in the cancer field related to cell-cycle dependent transcriptional events tha
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We report two cell synchronization protocols that provide a context for studying events related to specific phases of the cell cycle. We show that this approach is useful for analyzing the regulation of specific genes in an unperturbed cell cycle or upon exposure to agents affecting the cell cycle.