The method of giant patches combined with ion-selective microelectrodes is a versatile method used to detect and quantify ion fluxes. Specifically, it is used to measure the proton fluxes generated by the activity of the electro-neutral ion transp
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This manuscript describes the applications of proton-selective electrodes and patch clamping methods to measure the activity of proton transport systems. These methods overcome some limitations of techniques commonly used to study proton transport activity, such as moderate sensitivity, time resolution and insufficient intracellular milieu control.