Results: Representative Fluorescence Quantification Over Time
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Conclusion
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The overall goal of this procedure is to quantify plasma membrane repairability in muscular dystrophy patient cells. This method can help answer key questions in muscular dystrophy, such as what is the relationship between a given mutation and mem
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Cell membrane wounding via two-photon laser is a widely used method for assessing membrane resealing ability and can be applied to multiple cell types. Here, we describe a protocol for in vitro live-imaging of membrane resealing in dysferlinopathy patient cells following two-photon laser ablation.