PMC Isolation and Cultivation by Intraperitoneal Lavage
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Fluorometric Intracellular Free Calcium Concentration Measurement in PMCs
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Beta-hexosaminidase Release Measurement in PMCs
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Results: Beta-hexosaminidase Release Colorimetric Multi-well Measurements Performed with PMCs Isolated from Wild Type Mice
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Conclusion
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These methods can help answer key questions in the field of intracellular signaling on excitable cells, such as which ion channels are critical for immune cell activation and mediator release. The main advantages of these techniques are their rela
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Here, we present a protocol to isolate and cultivate murine peritoneal mast cells. We also describe two protocols for their functional characterization: a fluorescent imaging of intracellular free Ca2+ concentration and a degranulation assay based on colorimetric quantification of the released β-hexosaminidase.