Results: Representative Myoblast Nucleus Differentiation and Tracking Analyses
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Conclusion
Transcripción
Without protocol, it is possible to investigate the dynamic process of myoblast differentiation and myofiber formation, in particular nuclear positioning by exploiting live cell imaging. Live cell imaging of myoblast with foreign cell nuclei allow
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Skeletal muscle differentiation is a highly dynamic process, which particularly relies on nuclear positioning. Here, we describe a method to track nuclei movements by live cell imaging during myoblast differentiation and myotube formation and to perform a quantitative characterization of nuclei dynamics by extracting information from automatic tracking.