Name: evaluation of hemisphere lateralization with bilateral local field potential recording in secondary motor cortex of mice
Uploaded: 2019-07-31T12:30:00
Description: Watch this Scientific Journal Video about Evaluation of Hemisphere Lateralization with Bilateral Local Field Potential Recording in Secondary Motor Cortex of Mice at JoVE.com

This work was supported by grants from the National Natural Science Foundation of China (31771219, 31871170), the Science and Technology Division of Guangdong (2013KJCX0054), and the Natural Science Foundation of Guangdong Province (2014A030313418, 2014A030313440).

All animals were paired-housed under standard conditions (12 h light/dark, constant temperature environment, free access to food and water) according to the Chinese Ministry of Science and Technology Laboratory Animals Guidelines and experiments were approved by the local ethical committee of Guangzhou University. This is a non-survival procedure.&#160;
NOTE: For data shown in the representative results, APP/PS1 (B6C3-Tg (APPswe, PSEN1dE9) 85Dbo/J) double-transgenic mice and littermate wild-ty...

We report here the procedure for in vivo bilateral extracellular recording, along with analyzing the synchronization of dual-region LFP signals, which is both flexible and easy to conduct for estimating brain hemisphere lateralization, as well as the connectivity, directionality or coupling between neural activities of two brain areas. This can be widely used to reveal not only group-neuronal activities, but also some basic properties of interregional electrophysiology, especially for labs which are interested i...

Alzheimer&#39;s disease (AD) is the most common form of dementia1,2. Extracellular beta amyloid protein (&#946;-amyloid protein, A&#946;) deposition and intracellular neurofibrillary tangles (NFTs) are the main pathological features of AD3,4,5, but the mechanisms underlying AD pathogenesis remain largely unclear. Cerebral cortex, a key structure in cognition and memory, is impaired in AD6, and motor deficits such as slow walking, difficulty navigating the environment and gait disturbances occur with advancing age7. A&#946; deposition and neurofibrillary tangles have also been observed in the premotor cortex (PMC) and supplementary motor area (SMA) in AD patients8 and cognitively impacted older adults9, indicating the involvement of an impaired motor system in AD pathogenesis.
The brain is formed by two distinct cerebral hemispheres that are divided by a longitudinal fissure. A healthy brain exhibits both structural and functional asymmetries10, which is called &#34;lateralization&#34;, allowing the brain to efficiently deal with multiple tasks and activities. Aging results in a deterioration in cognition and locomotion, together with a reduction in brain laterality11,12. The motor abilities of the left hemisphere are readily apparent in the healthy brain13, but in the AD brain aberrant laterality occurs as a consequence of the failure of left hemisphere dominance associated with left cortical atrophy14,15,16. Therefore, an understanding of a possible alteration of brain lateralization in AD pathogenesis and the underlying mechanisms may provide new insights into AD pathogenesis and lead to identification of potential biomarkers for treatment.
Electrophysiological measurement is a sensitive and effective method of evaluating changes in the neuronal activities of animals. The reduction of hemispheric asymmetry in elders (HAROLD)17 has been documented by electrophysiological research with synchronized interhemispheric transfer time, which shows weakening or absence of hemispheric asymmetry to monaurally presented speech stimuli in the elderly18. Utilizing APP/PS1, one of the most commonly used AD mouse models19,20,21,22, in combination with in vivo bilateral extracellular recording of LFPs in both left and right M2, we evaluated possible laterality deficits in AD. In addition, with simple parameter settings, the built-in function of data analysis software (see the Table of Materials) provides a faster and more straightforward way to analyze the synchronization of electrical signals than mathematically complex programming language, which is friendly to beginners with in vivo electrophysiology.

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evaluation of hemisphere lateralization with bilateral local field potential recording in secondary motor cortex of mice

This article demonstrates complete, detailed procedures for both in vivo bilateral recording and analysis of local field potential (LFP) in the cortical areas of mice, which are useful for evaluating possible laterality deficits, as well as for assessing brain connectivity and coupling of neural network activities in rodents. The pathological mechanisms underlying Alzheimer&#39;s disease (AD), a common neurodegenerative disease, remain largely unknown. Altered brain laterality has been demonstrated in aging people, but whether or not abnormal lateralization is one of the early signs of AD has not been determined. To investigate this, we recorded bilateral LFPs in 3-5-month-old AD model mice, APP/PS1, together with littermate wild type (WT) controls. The LFPs of the left and right secondary motor cortex (M2), specifically in the gamma band, were more synchronized in APP/PS1 mice than in WT controls, suggesting a declined hemispheric asymmetry of bilateral M2 in this AD mouse model. Notably, the recording and data analysis processes are flexible and easy to carry out, and can also be applied to other brain pathways when conducting experiments that focus on neuronal circuits.

To see whether early AD pathology impairs the capacity of hemisphere lateralization, we conducted bilateral extracellular LFP recordings in the left and right M2 of APP/PS1 mice and WT controls (aged 3-5 months), and analyzed the cross-correlation of these left and right LFPs. In WT mice, the results demonstrated that the mean correlation between left and right LFPs at positive time lags differed significantly from that at negative time lags, implicating the existence of hemispheric asymmetries in M2 areas of WT controls...

Watch this Scientific Journal Video about Evaluation of Hemisphere Lateralization with Bilateral Local Field Potential Recording in Secondary Motor Cortex of Mice at JoVE.com

Evaluation of Hemisphere Lateralization with Bilateral Local Field Potential Recording in Secondary Motor Cortex of Mice

We present in vivo electrophysiological recording of the local field potential (LFP) in bilateral secondary motor cortex (M2) of mice, which can be applied to evaluate hemisphere lateralization. The study revealed altered levels of synchronization between the left and right M2 in APP/PS1 mice compared to WT controls.

This article demonstrates complete, detailed procedures for both in vivo bilateral recording and analysis of local field potential (LFP) in the cortical ...

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