Name: determining the functional status of the corticospinal tract within one week of stroke
Uploaded: 2020-02-22T13:00:00
Description: Watch this Scientific Journal Video about Determining the Functional Status of the Corticospinal Tract Within One Week of Stroke at JoVE.com

The authors thank Professor Winston Byblow and Harry Jordan for their valuable contribution to this work. This work was funded by the Health Research Council of New Zealand.

All research conducted with human participants must have human ethics approval by the appropriate institutional ethics committee and the study must be conducted in accordance with the declaration of Helsinki.
1. Patient Screening
<ol>
	<li>Screen all patients for PREP2 suitability within 72 h of stroke onset. 
	NOTE: Patients are suitable if they have had a unilateral ischemic or hemorrhagic stroke within the last 72 h, have new UL weakness, and are 18 years or older.</li>
</ol>
<p clas...

<ol>
	<li>Veerbeek, J. M., Kwakkel, G., van Wegen, E. E., Ket, J. C., Heymans, M. W. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Early+prediction+of+outcome+of+activities+of+daily+living+after+stroke:+a+systematic+review.">Early prediction of outcome of activities of daily living after stroke: a systematic review.</a> Stroke. 42 (5), 1482-1488 (2011).</li><li>Lohse, K. R., Schaefer, S. Y., Raikes, A. C., Boyd, L. A., Lang, C. E. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Asking+New+Questions+with+Old+Data:+The+Centralized+Open-Access+Rehabilitation+Database+for+Stroke.">Asking New Questions with Old Data: The Centralized Open-Access Rehabilitation Database for Stroke.</a> Frontiers in Neurology. 7, 153 (2016).</li><li>Stinear, C., Ackerley, S., Byblow, W. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Rehabilitation+is+initiated+early+after+stroke,+but+most+motor+rehabilitation+trials+are+not:+a+systematic+review.">Rehabilitation is initiated early after stroke, but most motor rehabilitation trials are not: a systematic review.</a> Stroke. 44 (7), 2039-2045 (2013).</li><li>Stinear, C. M. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Prediction+of+recovery+of+motor+function+after+stroke.">Prediction of recovery of motor function after stroke.</a> Lancet Neurology. 9 (12), 1228-1232 (2010).</li><li>Byblow, W. D., Stinear, C. M., Barber, P. A., Petoe, M. A., Ackerley, S. J. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Proportional+recovery+after+stroke+depends+on+corticomotor+integrity.">Proportional recovery after stroke depends on corticomotor integrity.</a> Annals of Neurology. 78 (6), 848-859 (2015).</li><li>Stinear, C. M. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Prediction+of+motor+recovery+after+stroke:+advances+in+biomarkers.">Prediction of motor recovery after stroke: advances in biomarkers.</a> Lancet Neurology. 16 (10), 826-836 (2017).</li><li>Kim, B., Winstein, C. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Can+Neurological+Biomarkers+of+Brain+Impairment+Be+Used+to+Predict+Poststroke+Motor+Recovery?+A+Systematic+Review.">Can Neurological Biomarkers of Brain Impairment Be Used to Predict Poststroke Motor Recovery? A Systematic Review.</a> Neurorehabilitation and Neural Repair. 31 (1), 3-24 (2016).</li><li>Boyd, L. A., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Biomarkers+of+stroke+recovery:+Consensus-based+core+recommendations+from+the+Stroke+Recovery+and+Rehabilitation+Roundtable.">Biomarkers of stroke recovery: Consensus-based core recommendations from the Stroke Recovery and Rehabilitation Roundtable.</a> International Journal of Stroke. 12 (5), 480-493 (2017).</li><li>Escudero, J. V., Sancho, J., Bautista, D., Escudero, M., Lopez-Trigo, J. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Prognostic+value+of+motor+evoked+potential+obtained+by+transcranial+magnetic+brain+stimulation+in+motor+function+recovery+in+patients+with+acute+ischemic+stroke.">Prognostic value of motor evoked potential obtained by transcranial magnetic brain stimulation in motor function recovery in patients with acute ischemic stroke.</a> Stroke. 29 (9), 1854-1859 (1998).</li><li>Pennisi, G., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Absence+of+response+to+early+transcranial+magnetic+stimulation+in+ischemic+stroke+patients:+prognostic+value+for+hand+motor+recovery.">Absence of response to early transcranial magnetic stimulation in ischemic stroke patients: prognostic value for hand motor recovery.</a> Stroke. 30 (12), 2666-2670 (1999).</li><li>Rapisarda, G., Bastings, E., de Noordhout, A. M., Pennisi, G., Delwaide, P. J. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Can+motor+recovery+in+stroke+patients+be+predicted+by+early+transcranial+magnetic+stimulation?.">Can motor recovery in stroke patients be predicted by early transcranial magnetic stimulation?.</a> Stroke. 27 (12), 2191-2196 (1996).</li><li>Bembenek, J. P., Kurczych, K., Karli Nski, M., Czlonkowska, A. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=The+prognostic+value+of+motor-evoked+potentials+in+motor+recovery+and+functional+outcome+after+stroke+-+a+systematic+review+of+the+literature.">The prognostic value of motor-evoked potentials in motor recovery and functional outcome after stroke - a systematic review of the literature.</a> Functional Neurology. 27 (2), 79-84 (2012).</li><li>Smania, N., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Active+finger+extension:+a+simple+movement+predicting+recovery+of+arm+function+in+patients+with+acute+stroke.">Active finger extension: a simple movement predicting recovery of arm function in patients with acute stroke.</a> Stroke. 38 (3), 1088-1090 (2007).</li><li>Nijland, R. H., van Wegen, E. E., Harmeling-van der Wel, B. C., Kwakkel, G. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=EPOS+Investigators.+Presence+of+finger+extension+and+shoulder+abduction+within+72+hours+after+stroke+predicts+functional+recovery:+early+prediction+of+functional+outcome+after+stroke:+the+EPOS+cohort+study.">EPOS Investigators. Presence of finger extension and shoulder abduction within 72 hours after stroke predicts functional recovery: early prediction of functional outcome after stroke: the EPOS cohort study.</a> Stroke. 41 (4), 745-750 (2010).</li><li>Katrak, P., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Predicting+upper+limb+recovery+after+stroke:+the+place+of+early+shoulder+and+hand+movement.">Predicting upper limb recovery after stroke: the place of early shoulder and hand movement.</a> Archives of Physical Medicine and Rehabilitation. 79 (7), 758-761 (1998).</li><li>Stinear, C. M., Barber, P. A., Petoe, M., Anwar, S., Byblow, W. D. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=The+PREP+algorithm+predicts+potential+for+upper+limb+recovery+after+stroke.">The PREP algorithm predicts potential for upper limb recovery after stroke.</a> Brain. 135 (Pt 8), 2527-2535 (2012).</li><li>Stinear, C. M., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=PREP2:+A+biomarker-based+algorithm+for+predicting+upper+limb+function+after+stroke.">PREP2: A biomarker-based algorithm for predicting upper limb function after stroke.</a> Annals of Clinical and Translational Neurology. 4 (11), 811-820 (2017).</li><li>Groppa, S., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=A+practical+guide+to+diagnostic+transcranial+magnetic+stimulation:+report+of+an+IFCN+committee.">A practical guide to diagnostic transcranial magnetic stimulation: report of an IFCN committee.</a> Clinical Neurophysiology. 123 (5), 858-882 (2012).</li><li>Barker, A. T., Jalinous, R., Freeston, I. L. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Non-invasive+magnetic+stimulation+of+human+motor+cortex.">Non-invasive magnetic stimulation of human motor cortex.</a> Lancet. 1 (8437), 1106-1107 (1985).</li><li>Stinear, C. M., Byblow, W. D., Ackerley, S. J., Barber, P. A., Smith, M. C. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Predicting+Recovery+Potential+for+Individual+Stroke+Patients+Increases+Rehabilitation+Efficiency.">Predicting Recovery Potential for Individual Stroke Patients Increases Rehabilitation Efficiency.</a> Stroke. 48 (4), 1011-1019 (2017).</li><li>Connell, L. A., Smith, M. C., Byblow, W. D., Stinear, C. M. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Implementing+biomarkers+to+predict+motor+recovery+after+stroke.">Implementing biomarkers to predict motor recovery after stroke.</a> NeuroRehabilitation. 43 (1), 41-50 (2018).</li><li>Smith, M. C., Ackerley, S. J., Barber, P. A., Byblow, W. D., Stinear, C. M. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=PREP2+Algorithm+Predictions+Are+Correct+at+2+Years+Poststroke+for+Most+Patients.">PREP2 Algorithm Predictions Are Correct at 2 Years Poststroke for Most Patients.</a> Neurorehabilitation and Neural Repair. 33 (8), 635-642 (2019).</li><li>Rossi, S., Hallett, M., Rossini, P. M., Pascual-Leone, A. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Safety,+ethical+considerations,+and+application+guidelines+for+the+use+of+transcranial+magnetic+stimulation+in+clinical+practice+and+research.">Safety, ethical considerations, and application guidelines for the use of transcranial magnetic stimulation in clinical practice and research.</a> Clinical Neurophysiology. 120 (12), 2008-2039 (2009).</li><li>Talelli, P., Greenwood, R. J., Rothwell, J. C. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Arm+function+after+stroke:+neurophysiological+correlates+and+recovery+mechanisms+assessed+by+transcranial+magnetic+stimulation.">Arm function after stroke: neurophysiological correlates and recovery mechanisms assessed by transcranial magnetic stimulation.</a> Clinical Neurophysiology. 117 (8), 1641-1659 (2006).</li></ol>

CST function evaluated with MEP status is a key prognostic biomarker for UL recovery and outcome after stroke. A total of 95% of patients with a functional CST at 1 week poststroke achieve an Action Research Arm Test (ARAT) score of at least 34 out of 57 by 3 months poststroke17. Conversely, 100% of patients without a functional CST at 1 week poststroke achieve an ARAT score of less than 34 by 3 months poststroke17. Evaluating CST function within a week poststroke may impro...

Upper limb function is commonly impaired after stroke, and recovery of UL function is important for regaining independence in daily living activities1. Stroke rehabilitation trials are often aimed at improving UL recovery and outcomes after stroke. The majority of stroke rehabilitation research is conducted with patients at the chronic stage (&#62;6 months poststroke), yet most rehabilitation occurs early after stroke2,3. More research needs to be conducted with patients soon after a stroke to build an evidence base for rehabilitation practice.
One of the greatest challenges when conducting research soon after the stroke is detecting the effects of the intervention against the background of recovery occurring during the initial weeks and months after the stroke. High intersubject variability in clinical presentation and recovery creates noise that can obscure the beneficial effects of interventions. Intervention and control groups are typically balanced on clinical measures of initial neurological impairment. However, these measures are often poor predictors of the patient&#39;s potential for subsequent recovery, particularly those with severe initial impairment4,5. This means that groups can be matched for baseline clinical measures and not matched for their recovery potential, which makes it more difficult to ascertain the intervention&#39;s effects. Biomarkers can address this challenge by identifying an individual patient&#39;s potential for motor recovery, so that groups can be accurately matched and stratified6,7,8. Biomarkers can also be used to select patients who are most likely to respond to the intervention&#39;s known or hypothesized mechanisms of action6.
The functional integrity of the corticospinal tract (CST) is a key biomarker that predicts recovery of UL function after stroke5,8,9,10,11,12. The CST conveys descending motor output from the primary motor cortex to the spinal cord and is essential for coordination and fine motor control. Patients with a functional CST after stroke are more likely to regain strength, coordination, and dexterity than patients without. A clinical assessment can be sufficient to confirm that the CST is functional in mildly impaired patients13,14,15. However, patients with more severe initial impairment may or may not have a functional CST, and a neurophysiological assessment using transcranial magnetic stimulation (TMS) is needed9,10,11,16,17.
TMS is a noninvasive and painless technique that can be used to test CST function18. The TMS coil delivers a magnetic stimulus over the primary motor cortex that generates a descending volley in the CST, eliciting a motor-evoked potential (MEP) in the muscles of the contralateral limb19. The presence of a MEP in the paretic arm or hand (MEP+) indicates a functional CST and is associated with greater potential for recovery of UL function. Patients who are MEP- are most likely to have worse UL recovery, with no return of coordinated and dexterous hand function4,6,9,12,16.
Testing all patients with TMS is impractical and unnecessary, as those with mild initial impairment most likely have a functional CST17. Therefore, a hierarchical approach is needed so that TMS is only used for patients with more severe initial impairment. The PREP2 algorithm was developed using a combination of clinical measures and TMS to evaluate CST function and predict likely UL outcome at 3 months poststroke (Figure 1)17. PREP2 starts at day 3 poststroke by testing the strength of shoulder abduction and finger extension in the paretic arm (SAFE score), using Medical Research Council grades. If the sum of these grades is 5 or more out of 10, it is &#34;safe&#34; to assume the patient is MEP+. These patients are expected to have a good or excellent UL outcome by 3 months poststroke, depending on their age17. These patients do not need TMS to determine MEP status, minimizing cost and unnecessary testing for the patient.
Patients with a SAFE score of less than 5 on day 3 poststroke require TMS to determine the functional integrity of their CST. If a MEP can be elicited from the paretic extensor carpi radialis (ECR) or first dorsal interosseus (FDI) muscles, the patient is MEP+ and is expected to recover fine motor control of the hand by 3 months poststroke. Approximately half of patients with a SAFE score less than 5 on day 3 poststroke are MEP+. Importantly, patients can have a SAFE score as low as zero and be MEP+. This illustrates the need for TMS in this subgroup of patients, as clinical assessment alone cannot distinguish between patients with and without a functional CST. Patients who are MEP- have significant CST damage. These patients are expected to have a limited or poor UL functional outcome depending on their overall stroke severity, measured with the National Institute of Health Stroke Scale (NIHSS) (Figure 1)17. These MEP- patients are not expected to regain coordinated and dexterous finger control and can be grouped together for research purposes.
<img alt="Figure 1" class="xfigimg" src="/files/ftp_upload/60665/60665fig01.jpg" /> 
Figure 1: The PREP2 algorithm. SAFE = Shoulder Abduction, Finger Extension score, which is the sum of the Medical Research Council grades for each of these movements out of 5, for a total SAFE score out of 10. MEP+ = Motor Evoked Potentials can be elicited from the paretic extensor carpi radialis (ECR) and/or first dorsal interosseous (FDI) muscles of the paretic UL using transcranial magnetic stimulation. NIHSS = National Institutes of Health Stroke Scale. The algorithm predicts one of four possible UL functional outcomes at 3 months poststroke. Each prediction category is associated with a rehabilitation focus that can be used to tailor UL therapy2. The colored dots represent, proportionally, PREP2 algorithm accuracy. The dots are color-coded based on the outcome category actually achieved 3 months poststroke (Green = Excellent; Blue = Good; Orange = Limited; Red = Poor). Figure reproduced from Stinear et al.17. <a href="https://www.jove.com/files/ftp_upload/60665/60665fig01large.jpg" target="_blank">Please click here to view a larger version of this figure.</a>
In clinical practice, PREP2 predicts one of four outcome categories that can be used to tailor rehabilitation for individual patients and help patients and families to understand what they can expect for their UL recovery. To date, PREP2 is the only externally validated UL prediction tool that combines clinical assessment and biomarker information in a decision tree17. It is also the only UL prediction tool with research on the effects of implementation in clinical practice20,21. PREP2 predictions are accurate for about 75% of patients, too optimistic for 17% and too pessimistic for 8% of patients at 3 months poststroke17. Accuracy is highest for MEP- patients (accurate for 90% of MEP- patients), highlighting the value of using TMS to identify these patients with severe damage to the descending motor pathways17. PREP2 remains correct for around 80% of patients at 2 years poststroke22. This supports the use of PREP2 to predict UL functional motor outcomes at 3 months and longer term. Information about delivering PREP2 predictions and using them in clinical practice is outside the scope of this methods paper, but detailed resources are available online23.
PREP2 provides researchers with a tool to select and stratify patients for clinical trials. This allows patients to be grouped not only according to baseline clinical characteristics, but also their neurobiological potential for UL recovery. Despite the mounting evidence for the use of TMS as a prognostic biomarker for UL recovery, lack of familiarity with TMS protocols in hospital settings with subacute stroke patients may be a barrier to its use in research. Therefore, this protocol aims to demonstrate how to use the SAFE score and TMS to evaluate CST function in patients in a hospital setting early after stroke.

<table border="1" fo:keep-together.within-page="1" fo:keep-with-next.within-page="always">
	<tbody>
		<tr>
			<td>alcohol/skin cleansing wipes</td>
			<td>Reynard</td>
			<td></td>
			<td>alcohol prep pads</td>
		</tr>
		<tr>
			<td>electromyography electrodes</td>
			<td>3M</td>
			<td></td>
			<td>red dot electrodes</td>
		</tr>
		<tr>
			<td>Magstim TMS coil</td>
			<td>Magstim</td>
			<td></td>
			<td>flat figure-8 coil</td>
		</tr>
		<tr>
			<td>razors</td>
			<td>any</td>
			<td></td>
			<td></td>
		</tr>
		<tr>
			<td>skin prep tape</td>
			<td>3M</td>
			<td></td>
			<td>red dot skin prep tape</td>
		</tr>
		<tr>
			<td>TMS stimulator</td>
			<td>Magstim</td>
			<td></td>
			<td>Magstim 200 single pulse stimulator</td>
		</tr>
	</tbody>
</table>

determining the functional status of the corticospinal tract within one week of stroke

High interindividual variability in the recovery of upper limb (UL) function after stroke means it is difficult to predict an individual&#39;s potential for recovery based on clinical assessments alone. The functional integrity of the corticospinal tract is an important prognostic biomarker for recovery of UL function, particularly for those with severe initial UL impairment. This article presents a protocol for evaluating corticospinal tract function within 1 week of stroke. This protocol can be used to select and stratify patients in trials of interventions designed to improve UL motor recovery and outcomes after stroke. The protocol also forms part of the PREP2 algorithm, which predicts UL function for individual patients 3 months poststroke. The algorithm sequentially combines a UL strength assessment, age, transcranial magnetic stimulation, and stroke severity, within a few days of the stroke. The benefits of using PREP2 in clinical practice are described elsewhere. This article focuses on the use of a UL strength assessment and transcranial magnetic stimulation to evaluate corticospinal tract function.

The SAFE score and TMS can be used to ascertain the functional status of the CST within one week of stroke. Patients who have a SAFE score of at least 5 on day 3, or are MEP+ when tested with TMS, have a functional CST and are expected to regain at least some coordination and dexterity. Patients who are MEP- do not have a functional CST and therefore are likely to be limited to improvements in proximal arm movements and gross movements of the hand. The functional status of the CST can therefore be used to select patients...

Watch this Scientific Journal Video about Determining the Functional Status of the Corticospinal Tract Within One Week of Stroke at JoVE.com

Determining the Functional Status of the Corticospinal Tract Within One Week of Stroke

This protocol is for evaluating corticospinal tract function within 1 week of stroke. It can be used to select and stratify patients in trials of interventions designed to improve upper limb motor recovery and outcomes and in clinical practice for predicting upper limb functional outcomes 3 months after stroke.

High interindividual variability in the recovery of upper limb (UL) function after stroke means it is difficult to predict an individual&#39;s potential ...

This protocol can be used to test a patient's corticospinal tract function within days of stroke in an acute hospital setting. Corticospinal tract function is an important predictor for upper limb recovery. The results of this test are useful for setting rehabilitation goals and selecting patients for rehabilitation trials.This protocol is part of the PREP2 algorithm which predicts upper limb outcomes after stroke. These predictions allow clinicians to personalize upper limb therapies and to manage patient expectations. Demonstrating the procedures will be Emma Monigatti and Ben Scrivener, physiotherapists, and Jim Stinear, a neuroscientist, who will play the role of the stroke patient.To assess the patient's SAFE score, position the patient with their back fully supported and upright and their paretic arm by their side with the elbow in extension. Demonstrate shoulder abduction and ask the patient to lift their arm sideways and up toward their ear. Use the medical research counsel grades to score the shoulder abduction strength.To score grades four or five, place a hand over the patient's arm proximal to the elbow and apply resistance. Next, place the paretic forearm in pronation with the fingers fully flexed and provide support under the wrist. Demonstrate finger extension and ask the patient to straighten their fingers.Use the medical research counsel grades, apply resistance over the dorsum of the fingers distal to the metacarpophalangeal joints throughout the movement to score grades four or five. If three fingers have the same score, use this score. If two fingers have a lower score than the other two fingers, use the lower score.Then add the grades for shoulder abduction and finger extension together for a SAFE score out of 10. For TMS, first remove any furniture from around the bed and move the bed away from the wall. Place the TMS unit at the head of the bed toward the side opposite the paretic limb and angle the TMS unit so that the screen could be easily observed.Remove any clothing covering the patient's paretic arm, including any items covering the wrist to allow EMG electrode placement. Position the paretic arm on a pillow with the forearm pronated and fully supported from the elbow to the hand and palpate the paretic forearm to locate the muscle belly of the ECR muscle. Identify positions for two surface EMG electrodes over the muscle belly allowing for factors such as the position of an IV cannula or dressings and use an alcohol skin cleansing wipe to clean the skin at each electrode site.Shave each site to remove any hair and lightly abrade the skin at both sites. Then securely apply self-adhesive disposable recording electrodes to each site. Next, locate the electrode sites for the FDI muscle and prepare the skin at the sites as just demonstrated to allow the placement of one electrode on the FDI muscle belly and one on the dorsum of the hand.Then place the reference electrode strap around the arm just proximal to the elbow. When the electrodes have been placed, lower the bed rails and move the patient as high up the bed as possible and toward the edge of the bed on the non-paretic side. Put the bed rails back up for safety and remove the headboard from the bed if possible.Remove any unused IV poles attached to the bed that may obstruct the coil position and raise the head of the bed as high as possible. Raise the knees if possible to prevent the patient from slipping down the bed during testing and position pillows behind the patient's back to bring the patient into an upright sitting position without the head contacting the bed. Ensure that the paretic forearm is in pronation and fully supported by a pillow from the elbow to the wrist and hold the TMS coil against the patient's head to check for adequate TMS coil access.When the patient is in position, connect the cables between the patient and the EMG unit and check to make sure that the EGM signal is free from any electrical noise. For TMS delivery, instruct the patient to look straight ahead while keeping their head still and their eyes open. Have one person hold the coil against the patient's head on the non-paretic side with the center of the coil positioned over the primary motor cortex of the stroke affected hemisphere approximately four centimeters lateral from the vertex on the interaural line.Orient the coil with the handle pointing backwards at an approximately 45 degree angle to the midsagittal plane to produce a posterior to anterior current in the underlying tissue and adjust the bed height for the coil holder's comfort. Have a second person monitor the patient for comfort throughout the TMS session. Begin with a stimulus intensity of 30%maximum stimulator output and increase the intensity in 10%output steps with three to five stimuli at each intensity and scalp location.Move the coil systematically in one centimeter steps in the anterior, posterior, medial, and lateral directions to find the optimal location for producing MEPs in the recorded muscles. Small adjustments to the coil rotation may also be necessary. Continue increasing the stimulus intensity and moving the coil until MEPs are consistently observed in one or both muscles or until 100%maximum stimulator output is reached.If a 100%maximum stimulator output is reached with no MEPs observed, ask the patient to hug a pillow to their chest with both arms to attempt to activate their paretic upper limb and increase the likelihood of eliciting a MEP. For patients with no distal upper limb activity, have the patient elevate and retract the shoulder girdle. Classify the patient as MEP positive if MEPs of any amplitude are observed with a consistent latency and response to at least five stimuli either at rest or during voluntary facilitation.FDI latencies are typically 20 to 30 milliseconds while ECR latencies are typically 15 to 25 milliseconds. MEPs do not have to exceed a peak-to-peak amplitude of 50 microvolts. Classify a patient as MEP negative if a MEP cannot be elicited at 100%maximum stimulator output either at rest or while attempting voluntary facilitation.This representative patient had MEPs in the paretic FDI and ECR muscles while this patient had a small MEP in the FDI muscle and no MEP in the ECR muscle. This patient did not have MEPs at a 100%maximum stimulator output while at rest or when attempting active bilateral facilitation. This patient had no MEP in the FDI muscle with an elongated tail of the stimulus artifact in the ECR muscle trace.When this artifact is present during the latency window for either muscle, the identification of a MEP can be difficult. This patient had MEPs in both muscles that are clearly identifiable despite the electrical noise in the FDI trace while this patient had MEPs only in the ECR. If the SAFE is less than five at three days post-stroke then TMS is required and should be completed within seven days post-stroke.The SAFE score and TMS evaluate corticospinal tract function and they can be combined in the PREP2 algorithm to predict upper limb outcomes at three months after stroke. This method allows the personalization of upper limb rehabilitation goals and the selection of patients in upper limb rehabilitation trials.

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Deriving the Time Course of Glutamate Clearance with a Deconvolution Analysis of Astrocytic Transporter Currents

The highest density of glutamate transporters in the brain is found in astrocytes. Glutamate transporters couple the movement of glutamate across the ...

Functional Analysis of the Larval Feeding Circuit in Drosophila

Functional Analysis of the Larval Feeding Circuit in Drosophila

The serotonergic  feeding circuit in Drosophila  melanogaster larvae can be used to investigate neuronal substrates of  critical importance during the ...

Unilateral Pyramidotomy of the Corticospinal Tract in Rats for Assessment of Neuroplasticity-inducing Therapies

The corticospinal tract (CST) can be completely severed unilaterally in the medullary pyramids of the rodent brainstem. The CST is a motor tract that has ...

External Excitation of Neurons Using Electric and Magnetic Fields in One- and Two-dimensional Cultures

A neuron will fire an action potential when its membrane potential exceeds a certain threshold. In typical activity of the brain, this occurs as a result ...

A Simple One-step Dissection Protocol for Whole-mount Preparation of Adult Drosophila Brains

A Simple One-step Dissection Protocol for Whole-mount Preparation of Adult Drosophila Brains

There is an increasing interest in using Drosophila to model human brain degenerative diseases, map neuronal circuitries in adult brains, and study the ...

Assay to Measure Nucleocytoplasmic Transport in Real Time within Motor Neuron-like NSC-34 Cells

Nucleocytoplasmic transport refers to the import and export of large molecules from the cell nucleus. Recently, a number of studies have shown a ...

Bilateral Assessment of the Corticospinal Pathways of the Ankle Muscles Using Navigated Transcranial Magnetic Stimulation

Distal leg muscles receive neural input from motor cortical areas via the corticospinal tract, which is one of the main motor descending pathway in humans ...

Using Zebrafish Larvae to Study the Pathological Consequences of Hemorrhagic Stroke

Despite being the most severe subtype of stroke with high global mortality, there is no specific treatment for patients with intracerebral hemorrhage ...

Measuring Post-Stroke Cerebral Edema, Infarct Zone and Blood-Brain Barrier Breakdown in a Single Set of Rodent Brain Samples

One of the most common causes of morbidity and mortality worldwide is ischemic stroke. Historically, an animal model used to stimulate ischemic stroke ...

Motor Dual-Tasks for Gait Analysis and Evaluation in Post-Stroke Patients

Eighteen stroke patients were recruited for this study involving the evaluation of cognition and walking ability and multitask gait analysis. Multitask ...