Cerebrospinal fluid leaks can either be caused by trauma, can pre-exist congenitally, or can appear spontaneously. In cases of CSF leaks, a reliable detection of its origin is needed in order to seal the leaks efficiently, and to prevent complicat
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Intrathecally applied fluorescein is used to achieve intraoperative visualization of CSF leaks. This protocol describes a lumbar puncture, the application of 5% fluorescein, and intraoperative visualization using a fully digital microscope.