Quantification of Circulating Pig-Specific DNA in the Blood of a Xenotransplantation Model

DOI :

10.3791/61579-v

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September 22nd, 2020

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3,829 Views

Yangyang Deng^1,2*, Ming Zhou^1,3*, Ying Lu¹, Jiao Chen¹, Zuhui Pu⁴, Dongjing Yu^1,5, Yifan Dai⁶, Yongqiang Zhan⁷, Lisha Mou¹

Chapters

In this protocol, porcine specific primers were designed, plasmids-containing porcine specific DNA fragments were constructed, and standard curves for quantitation were established. Using species-specific primers, cpsDNA was quantified by qPCR in pig-to-mouse cell transplantation models and pig-to-monkey artery patch transplantation models.

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