February 4th, 2021
•Presented here is a procedure to express and purify myosin 5a followed by a discussion of its characterization, using both ensemble and single molecule in vitro fluorescence microscopy-based assays, and how these methods can be modified for the characterization of nonmuscle myosin 2b.
Tags
Videos relacionados
Automated Acoustic Dispensing for the Serial Dilution of Peptide Agonists in Potency Determination Assays
Small-Scale Colorimetric Assays of Intracellular Lactate and Pyruvate in the Nematode Caenorhabditis elegans
Quantification of Hypopigmentation Activity In Vitro
Isolation of Physiologically Active Thylakoids and Their Use in Energy-Dependent Protein Transport Assays
Using In Vitro Fluorescence Resonance Energy Transfer to Study the Dynamics Of Protein Complexes at a Millisecond Time Scale
In Vitro Ubiquitination and Deubiquitination Assays of Nucleosomal Histones
A Planarian Motility Assay to Gauge the Biomodulating Properties of Natural Products
Time-resolved Förster Resonance Energy Transfer Assays for Measurement of Endogenous Phosphorylated STAT Proteins in Human Cells
Continuous Fluorescence-Based Endonuclease-Coupled DNA Methylation Assay to Screen for DNA Methyltransferase Inhibitors
In Vitro Characterization of Histone Chaperones using Analytical, Pull-Down and Chaperoning Assays
ACERCA DE JoVE
Copyright © 2024 MyJoVE Corporation. Todos los derechos reservados