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In Vitro Ubiquitination and Deubiquitination Assays of Nucleosomal Histones

DOI :

10.3791/59385-v

July 25th, 2019

July 25th, 2019

10,139 Views

1Maisonneuve-Rosemont Hospital Research Center and Department of Medicine, University of Montréal, 2Lunenfeld-Tanenbaum Research Institute, Sinai Health System

Ubiquitination is a post-translational modification that plays important roles in cellular processes and is tightly coordinated by deubiquitination. Defects in both reactions underlie human pathologies. We provide protocols for conducting ubiquitination and deubiquitination reaction in vitro using purified components.

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In Vitro

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