Results: Mature Megakaryocyte Differentiation and Platelet Formation
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Conclusion
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This protocol proposes a simple method for obtaining human hematopoietic progenitors, and for inducing their differentiation into megakaryocytes. It can serve as a basis for better understanding of megakaryopoiesis This technique uses a source, bu
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This protocol describes in detail all the steps involved in obtaining leukofilter-derived CD34+ hematopoietic progenitors and their in vitro differentiation and maturation into proplatelet-bearing megakaryocytes that are able to release platelets in the culture medium. This procedure is useful for in-depth analysis of cellular and molecular mechanisms controlling megakaryopoiesis.