Pooled shRNA Library Screening to Identify Factors that Modulate a Drug Resistance Phenotype

0:24

Selection of the Most Potent Promoter to Obtain Persistent and Prolonged Expression of the shRNAs

2:01

Preparation of Pooled Lentiviral Human Epigenetic Factor shRNA Library

4:48

Estimation of the Transduction Efficiency of Lentiviruses

6:03

Transduction of Pooled Epigenetic shRNA Library in the Drug-Resistant Cell Line

7:20

Enrichment of GFP Positive Cells

7:54

Dropout Screening to Identify Epigenetic Factors Mediating Drug Resistance

9:20

Amplification of the Integrated shRNAs by PCR

11:08

Next-Generation Sequencing and Data Analysis

11:57

Representative Results

14:30

Conclusion