Results: Whole‐Cell Patch‐Clamp Recordings on the Brain Slices
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By measuring the electrophysiological properties of cell membranes, the question of the main proves that modulate the electrical activity of a cell can be solved. Whole-cell patch clamp recording is a peripheral technique to study ionic currents i
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Here, we present a protocol to perform a whole-cell patch-clamp on brain slices containing kisspeptin neurons, the primary modulator of gonadotrophin-releasing hormone (GnRH) cells. By adding knowledge about kisspeptin neuron activity, this electrophysiological tool has served as the basis for significant advancements in the neuroendocrinology field over the last 20 years.