Isolation of Endophytic Fungi Associated with Plant Organs
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Preservation of Purified Fungal Isolates
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Micromorphological Characterization (Hyphal Morphology) of Filamentous Fungi
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Results: Isolation and Macromorphology Evaluation of Endophytic Fungi Associated with Mycoheterotrophic Plants
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Conclusion
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The techniques presented here are useful for investigating the endophytic fungi community associated with different plant organs. Endophytic fungi isolation allows their identification, and is valuable in other techniques such symbiotic germinatio
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The current article aims to provide detailed and adequate protocols for the isolation of plant-associated endophytic fungi, long-term preservation of isolates, morphological characterization, and total DNA extraction for subsequent molecular identification and metagenomic analyses.