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Measuring Diffusion Coefficients via Two-photon Fluorescence Recovery After Photobleaching

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February 26th, 2010

DOI :

10.3791/1636-v

February 26th, 2010

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1Department of Physics and Astronomy, University of Rochester, 2Department of Biomedical Engineering, University of Rochester

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Diffusion Coefficients
Two photon Fluorescence Recovery After Photobleaching
Multi fluorescence Recovery After Photobleaching
Microscopy Technique
Macromolecules
In Vitro
In Vivo
Biological Systems
Photobleaching
Laser Flash
Fluorescence Monitoring
Pockels Cell
Laser Modulator
Optical Path
Sample Preparation
Aqueous Fluorescent Dye
Experimental Parameters
Monitor Power
Bleaching Power
Bleach Duration
Bin Widths
Fluorescence Recovery Time
Recovery Curves
LabVIEW Automation
Enhanced Throughput
Mathematical Model Fitting Algorithm
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Measuring Diffusion Coefficients via Two-photon Fluorescence Recovery After Photobleaching

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