November 23rd, 2011
•The cytochrome c oxidase/sodium dehydrogenase (COX/SDH) double-labeling method allows for direct visualization of mitochondrial respiratory enzyme deficiencies in fresh-frozen tissue sections. This is a straightforward histochemical technique and is useful in investigating mitochondrial diseases, aging, and aging-related disorders.
Vidéos Connexes
Generation of Stable Transgenic C. elegans Using Microinjection Video (Video) | JoVE
Activation of Apoptosis by Cytoplasmic Microinjection of Cytochrome c Video (Video) | JoVE
The ex vivo Isolated Skeletal Microvessel Preparation for Investigation of Vascular Reactivity Video (Video) | JoVE
Assessment of Mitochondrial Functions and Cell Viability in Renal Cells Overexpressing Protein Kinase C Isozymes Video (Video) | JoVE
Analysis of Gene Function and Visualization of Cilia-Generated Fluid Flow in Kupffer's Vesicle Video (Video) | JoVE
Visualization of Craniofacial Development in the sox10: kaede Transgenic Zebrafish Line Using Time-lapse Confocal Microscopy Video (Video) | JoVE
Large-scale Gene Knockdown in C. elegans Using dsRNA Feeding Libraries to Generate Robust Loss-of-function Phenotypes Video (Video) | JoVE
Measuring Respiratory Function in Mice Using Unrestrained Whole-body Plethysmography Video (Video) | JoVE
Experimental Approaches to Study Mitochondrial Localization and Function of a Nuclear Cell Cycle Kinase, Cdk1 Video (Video) | JoVE
High-resolution Respirometry to Assess Mitochondrial Function in Permeabilized and Intact Cells Video (Video) | JoVE