S'identifier

JoVE Journal

Medicine

Author Produced

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Modeling Colitis-Associated Cancer with Azoxymethane (AOM) and Dextran Sulfate Sodium (DSS)

46.4K Views

12:37 min

September 11th, 2012

September 11th, 2012

46,373 Views

0:05

Title

1:26

Colitis-associated Cancer Induction

2:28

Clinical Assessment of Colitis and Tumor Progression

2:59

Murine Endoscopy(Optional)

4:24

Mouse Sacrifice and Colon Harvesting

6:55

Preparing the Colon for Macroscopic Analysis

7:55

Preparing the Colon for Histological Assessment

9:21

Representative Results

We demonstrate a protocol in which administration of the genotoxic agent azoxymethane (AOM) followed by three cycles of the pro-inflammatory agent dextran sulfate sodium (DSS) rapidly and consistently generates colon tumors in mice with morphologic and molecular similarities to those seen in human colitis-associated cancer.

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