Name: high throughput assay to examine egg-laying preferences of individual drosophila melanogaster
Uploaded: 2016-03-24T13:00:00.000+00:00
Duration: 3 min 58 s
Description: Watch this Scientific Journal Video about High Throughput Assay to Examine Egg-Laying Preferences of Individual Drosophila melanogaster at JoVE.com

We thank the Duke Physics Shop, especially Phil Lewis, for helping us build the behavioral apparatus and attachments and creating the drawing. This work is funded by the National Institutes of Health under award number R01GM100027.

1. Préparation de mouches à doser <ol><li> Culture vole sur la mélasse standards / media à la semoule de maïs incubateur réglé à 25 ° C et 65% d&#39;humidité. Prenez soin de ne pas surcharger les flacons. Par exemple, mettre 8 femmes et 6 hommes dans un flacon alimentaire étroite. Note: &quot;flacon alimentaire étroite» utilisée ici a un diamètre intérieur de 2,3 cm. Nous généralement distribué environ 10 ml de nourriture à la mouche dans chaque flacon. La recette de la nourriture à la mouche, nous avons utilisé est décrit ici: http://flystocks.bio.indiana.edu/Fly_Work/media-recipes/molassesfood.html. </li><li> 2 - 3 jours après les femelles eclosion, préparer des flacons avec de la pâte de levure fraîche comme le montre la figure 1E. Faire de la pâte de levure fraîche en mélangeant 6 g de levure active avec 10 ml d&#39;acide propionique 0,5%. Utiliser une spatule pour appliquer la pâte de levure sur la paroi latérale des flacons de produits alimentaires. Collecter 30 - 35 femelles éclos avec 20 - 25 mâles dans les flacons. <ol><li> En général, de recueillir les mâles des mêmes flacons où les femelles sont collectées(Afin de gagner du temps et de l&#39;effort). Toutefois, si l&#39;on se préoccupe de la fertilité des mâles, utilisez les mâles WT à la place. En outre, la pâte de levure est important pour stimuler la production d&#39;œufs donc le préparer frais tous les jours. </li></ol></li><li> Maintenir les hommes / femmes recueillies à 25 ° C, 65% d&#39;humidité, à moins que les mouches sont sensibles à la température. </li><li> Après ~ 4 - 5 jours, vérifiez les flacons pour voir si les femelles sont prêtes pour des expériences de ponte. Ils sont prêts lorsque la surface du milieu alimentaire est humide à partir de larves fouisseuses activement à la nourriture (voir la figure 1E - F). Remarque: Les femelles retiennent généralement pondre une fois que la surface de l&#39;aliment devient humide et grouillante de larves. Cette étape garantit que les femmes seraient prêtes à pondre d&#39;œufs quand ils rencontrent des substrats avec la texture désirée (par exemple., ~ 1% d&#39; agarose). </li></ol> 2. Chambre de la Construction, Assemblée, Assay Setup <ol><li> Avoir un atelier d&#39;usinage de construire des chambres de ponte acryliques (Figurer 1A - D). Le dessin technique de différentes pièces ont été montrés à la figure supplémentaire 1 -. 3 images haute résolution peuvent également être trouvés ici (http://www.rebeccayang.org/pdf/chamber%20design.pdf). </li><li> Insérez des feuilles de plastique dans le chargement ( en haut) morceau de la chambre comme le montre la figure 1C. Cela sert de surface de fond pendant le chargement vole dans les arènes de ponte individuels. </li><li> Anesthetize femelles sur un bloc de CO 2 et de les charger individuellement dans chaque arène de ponte. Autoriser ~ 30 min pour les mouches pour récupérer du CO 2 et de s&#39;acclimater à ce nouvel environnement. </li><li> Préparer les substrats d&#39;agarose. <ol><li> Pour plus de commodité, garder une bouteille de premade fondu 1% d&#39;agarose dans un bain d&#39;eau C 55 °. </li><li> Ajouter la quantité désirée de solution mère de saccharose (2 M) dans un tube conique de 50 ml et en le mélangeant avec une quantité appropriée d&#39;agarose. Par exemple, pour préparer le substrat mM, saccharose 150, plaCE 750 pi de solution 2 M de saccharose dans le tube, puis remplir le tube avec de l&#39;agarose à la marque 10 ml. </li><li> Préparer le substrat brut de la même manière mais en ajoutant de l&#39;eau distillée au lieu de la solution de saccharose. Remarque: La concentration finale d&#39;agarose dans ce protocole est légèrement inférieur à 1%. Dans notre expérience, la concentration exacte de l&#39;agarose n&#39;a pas d&#39;importance tant qu&#39;il est commandée pour être à l&#39;intérieur de ~ 0,9 à 1,1% et que les deux substrats sont de la même concentration d&#39;agarose. </li></ol></li><li> Prendre la pièce de la chambre et la pipette de 1000 ul de substrat d&#39; agarose substrat ( en bas) dans chaque creux comme on le voit sur ​​la figure 1D. </li><li> Laisser agarose se solidifier pendant ~ 30 min. </li><li> Une fois que les substrats d&#39;agarose et les mouches sont prêts, assembler les trois morceaux de la chambre de ponte et ensuite prendre les feuilles de plastique. </li><li> Placer les chambres dans les incubateurs de mouches. Note: Longueur des expériences de ponte peut varier selon expebesoins rimentales. Nous courons généralement l&#39;expérience O / N (14 - 16 h). En outre, on a observé aucun impact significatif de la synchronisation circadienne sur les préférences de ponte. </li><li> Anesthetize femelles par injection de CO 2 dans la chambre. Démonter la chambre, jeter les mouches anesthésiés dans la mouche morgue (ie., Un café vide peut rempli avec de l&#39; huile de maïs). Prenez des photos des résultats pour la tenue des dossiers (voir la figure 2). </li><li> Comptez le nombre d&#39;oeufs manuellement et calculer les indices de préférence pour l&#39;analyse. Calculer l&#39; indice de préférence en tant que (N a - N b) / (N a + N b) , où N et N a b représentent le nombre d&#39;oeufs sur le site d&#39; un site par rapport à b, respectivement. </li></ol>

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The authors have nothing to disclose.

Les chambres et les protocoles décrits ici ont plusieurs améliorations par rapport à des essais de ponte précédents. Tout d&#39;abord, ils augmentent le débit de dosage préférences des animaux isolés de manière significative. Chaque chambre peut doser 30 femmes célibataires et il faut moins d&#39;une heure à mettre en place. D&#39;autre part, ils augmentent la cohérence des préférences de ponte par rapport aux méthodes antérieures. La normalisation des dimensions de l&#39;arène, la taille des substrats...

Drosophila melanogaster is a powerful genetic model organism to study the neural basis of behaviors. The rapid developments of genetic tools to manipulate neurons in a targeted manner and the emergence of sophisticated behavioral analysis tools have significantly improved our ability to dissect the circuit mechanisms that underlie the sensory-motor transformation processes of several innate and learned behaviors1-3.
Drosophila egg-laying is a suitable model to study the neural basis of simple decision making processes. In particular, Drosophila females have been shown to possess the ability to compare and rank their options before "committing" to depositing an egg onto a given option4-8. For example, when given only a plain (sucrose-free) substrate or only a sucrose-containing substrate, females readily accept either option for egg-laying. However, when presented with both options, females robust reject the sucrose substrate in some contexts7,9,10. Relatively little is known about the neural mechanisms that allow females to "choose the greater of two goods", however. A major obstacle has been the lack of an efficient method to assay egg-laying preferences such that one can use a systematic genetic screening approach to study this problem.
In this report, we describe the protocol we developed that allows egg-laying preferences of females to be assayed at single-animal resolution and with substantially improved throughput and consistency over previous methods. Specifically, we provide the blueprints for constructing the chambers we designed, the protocol for preparing the females so that each is primed to lay many eggs, and the protocol for using the chambers.

<table><tbody><tr><td>UltraPure Agarose&amp;nbsp;</td><td>Invitrogen</td><td>16500-500</td><td></td></tr><tr><td>Sucrose</td><td>Sigma</td><td>S0389</td><td></td></tr><tr><td>Water bath&amp;nbsp;</td><td>Fisher</td><td>15-462-6Q</td><td></td></tr><tr><td>LifeCam Cinema webcam</td><td>Microsoft</td><td>H5D-00013</td><td></td></tr><tr><td>Red LEDs</td><td>Cree</td><td>C503B-RAN-CA0B0AA1</td><td></td></tr><tr><td>Egg-laying chambers</td><td>Custom Built</td><td></td><td></td></tr><tr><td>Camera holders</td><td>Custom Built</td><td></td><td></td></tr><tr><td>LED holders</td><td>Custom Built</td><td></td><td></td></tr><tr><td>Fly vials (narrow)</td><td>Genesee</td><td>32-116BC</td><td></td></tr></tbody></table>

high throughput assay to examine egg-laying preferences of individual drosophila melanogaster

Récemment, la préférence de la drosophile ponte a émergé comme un modèle génétiquement traitable pour étudier les bases neurales des processus de prise de décisions simples. Lors de la sélection des sites pour déposer leurs œufs, les mouches femelles sont capables de classer l&#39;attractivité relative de leurs options et en choisissant les &quot;plus de deux produits.&quot; Cependant, la plupart des essais de préférence ponte ne sont pas pratiques si l&#39;on veut adopter une approche de dépistage génétique systématique à la recherche de la base de circuits qui sous-tend ce processus de prise de décision simple, comme ils sont et laborieux à mettre en place basée sur la population. Pour augmenter le débit de l&#39;étude des préférences de ponte des femelles simples, nous avons développé personnalisés chambres que chacun peut les préférences de ponte simultanément dosage allant jusqu&#39;à trente individu vole ainsi qu&#39;un protocole qui assure chaque femelle a un taux de ponte élevé (de sorte que leur préférence est facilement discernable et plus convaincante). Notre approche est simple à exécuteret produit des résultats très cohérents. En outre, ces chambres peuvent être équipées de différents accessoires pour permettre l&#39;enregistrement vidéo des animaux de ponte et de fournir de la lumière pour les études de optogénétique. Cet article fournit les plans pour la fabrication de ces chambres et la procédure pour la préparation des mouches à doser dans ces chambres.

Les chambres de ponte sont composés de plusieurs pièces: un morceau substrat ( en bas), un diviseur (milieu) pièce, une charge ( en haut) pièce, et 2 portes coulissantes (Figure 1A - D). Ces pièces sont utilisées pour indépendamment les mouches de configuration et des substrats avant des expériences de ponte. Figure 1F montre comment les flacons devraient regarder quand mouches femelles sont prêtes pour la ponte. Quand les mouches ont le choix e...

Watch this Scientific Journal Video about High Throughput Assay to Examine Egg-Laying Preferences of Individual Drosophila melanogaster at JoVE.com

High Throughput Assay to Examine Egg-Laying Preferences of Individual Drosophila melanogaster

High Throughput Assay pour examiner les préférences de ponte de l&#39;individu<em&gt; Drosophila melanogaster</em

This protocol describes a high throughput assay for testing egg-laying preferences of Drosophila melanogaster at single-animal resolution. This assay provides a simple, efficient, and scalable platform to identify genes and circuit components that control a simple decision-making process.

Recently, egg-laying preference of Drosophila has emerged as a genetically tractable model to study the neural basis of simple decision-making processes. ...

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Research

JoVE Journal

Neuroscience

High Throughput Assay to Examine Egg-Laying Preferences of Individual Drosophila melanogaster

7.9K vues.  Duke University. This protocol describes a high throughput assay for testing egg-laying preferences of Drosophila melanogaster at single-animal resolution. This assay provides a simple, efficient, and scalable platform to identify genes and circuit components that control a simple decision-making process.

Vidéo: High Throughput Assay to Examine Egg-Laying Preferences of Individual Drosophila melanogaster

Methods to Assay Drosophila Behavior

Drosophila melanogaster, the fruit fly, has been used to study molecular mechanisms of a wide range of human diseases such as cancer, cardiovascular disease and various neurological diseases1. We have optimized simple and robust behavioral assays for determining larval locomotion, adult climbing ability (RING assay), and courtship behaviors of Drosophila. These behavioral assays are widely applicable for studying the role of genetic and environmental factors on fly behavior. Larval crawling ability can be reliably used for determining early stage changes in the crawling abilities of Drosophila larvae and also for examining effect of drugs or human disease genes (in transgenic flies) on their locomotion. The larval crawling assay becomes more applicable if expression or abolition of a gene causes lethality in pupal or adult stages, as these flies do not survive to adulthood where they otherwise could be assessed. This basic assay can also be used in conjunction with bright light or stress to examine additional behavioral responses in Drosophila larvae. Courtship behavior has been widely used to investigate genetic basis of sexual behavior, and can also be used to examine activity and coordination, as well as learning and memory. Drosophila courtship behavior involves the exchange of various sensory stimuli including visual, auditory, and chemosensory signals between males and females that lead to a complex series of well characterized motor behaviors culminating in successful copulation. Traditional adult climbing assays (negative geotaxis) are tedious, labor intensive, and time consuming, with significant variation between different trials2-4. The rapid iterative negative geotaxis (RING) assay5 has many advantages over more widely employed protocols, providing a reproducible, sensitive, and high throughput approach to quantify adult locomotor and negative geotaxis behaviors. In the RING assay, several genotypes or drug treatments can be tested simultaneously using large number of animals, with the high-throughput approach making it more amenable for screening experiments.

Methods to Assay Drosophila Behavior

Drosophila melanogaster is a genetically and behaviorally tractable model system that has been used to understand the molecular and cellular basis of many important biological processes for over a century 1. Drosophila has been well exploited to gain insights into the genetic basis of fly behavior.

Méthodes de dosage à Drosophila Comportement

Drosophila melanogaster, the fruit fly, has been used to study molecular mechanisms of a wide range of human diseases such as cancer, cardiovascular ...

Recherche

Neurosciences

Light Preference Assay to Study Innate and Circadian Regulated Photobehavior in Drosophila Larvae

Light acts as environmental signal to control animal behavior at various levels. The Drosophila larval nervous system is used as a unique model to answer basic questions on how light information is processed and shared between rapid and circadian behaviors. Drosophila larvae display a stereotypical avoidance behavior when exposed to light. To investigate light dependent behaviors comparably simple light-dark preference tests can be applied. In vertebrates and arthropods the neural pathways involved in sensing and processing visual inputs partially overlap with those processing photic circadian information. The fascinating question of how the light sensing system and the circadian system interact to keep behavioral outputs coordinated remains largely unexplored. Drosophila is an impacting biological model to approach these questions, due to a small number of neurons in the brain and the availability of genetic tools for neuronal manipulation. The presented light-dark preference assay allows the investigation of a range of visual behaviors including circadian control of phototaxis.

Light Preference Assay to Study Innate and Circadian Regulated Photobehavior in Drosophila Larvae

Here we describe a light-dark preference test for Drosophila larva. This assay provides information about innate and circadian regulation of light sensing and processing photobehavior.

Essai de préférence la lumière pour étudier innée et circadien Photobehavior Réglementé<em&gt; Drosophila</em&gt; Les larves

Light acts as environmental signal to control animal behavior at various levels. The Drosophila larval nervous system is used as a unique model to answer ...

A Single-fly Assay for Foraging Behavior in Drosophila

For many animals, hunger promotes changes in the olfactory system in a manner that facilitates the search for appropriate food sources. In this video article, we describe an automated assay to measure the effect of hunger or satiety on olfactory dependent food search behavior in the adult fruit fly Drosophila melanogaster. In a light-tight box illuminated by red light that is invisible to fruit flies, a camera linked to custom data acquisition software monitors the position of six flies simultaneously. Each fly is confined to walk in individual arenas containing a food odor at the center. The testing arenas rest on a porous floor that functions to prevent odor accumulation. Latency to locate the odor source, a metric that reflects olfactory sensitivity under different physiological states, is determined by software analysis. Here, we discuss the critical mechanics of running this behavioral paradigm and cover specific issues regarding fly loading, odor contamination, assay temperature, data quality, and statistical analysis.

A Single-fly Assay for Foraging Behavior in Drosophila

In this video article, we describe an automated assay to measure the effect of hunger or satiety on olfactory dependent food search behavior in the adult fruit fly Drosophila melanogaster.

Une simple mouche Essai de comportement alimentaire dans<em&gt; Drosophila</em

For many animals, hunger promotes changes in the olfactory system in a manner that facilitates the search for appropriate food sources. In this video ...

Assay for Blood-brain Barrier Integrity in Drosophila melanogaster

Proper nervous system development includes the formation of the blood-brain barrier, the diffusion barrier that tightly regulates access to the nervous system and protects neural tissue from toxins and pathogens. Defects in the formation of this barrier have been correlated with neuropathies, and the breakdown of this barrier has been observed in many neurodegenerative diseases. Therefore, it is critical to identify the genes that regulate the formation and maintenance of the blood-brain barrier to identify potential therapeutic targets. In order to understand the exact roles these genes play in neural development, it is necessary to assay the effects of altered gene expression on the integrity of the blood-brain barrier. Many of the molecules that function in the establishment of the blood-brain barrier have been found to be conserved across eukaryotic species, including the fruit fly, Drosophila melanogaster. Fruit flies have proven to be an excellent model system for examining the molecular mechanisms regulating nervous system development and function. This protocol describes a step-by-step procedure to assay for blood-brain barrier integrity during the embryonic and larval stages of D. melanogaster development.

Assay for Blood-brain Barrier Integrity in Drosophila melanogaster

Blood-brain barrier integrity is critical for nervous system function. In Drosophila melanogaster, the blood-brain barrier is formed by glial cells during late embryogenesis. This protocol describes methods to assay for blood-brain barrier formation and maintenance in D. melanogaster embryos and third instar larvae.

Analyse de l'intégrité de la barrière hémato-encéphalique dans le mélanogaster Drosophila

Proper nervous system development includes the formation of the blood-brain barrier, the diffusion barrier that tightly regulates access to the nervous ...

Biologie du développement

A Behavioral Assay for Mechanosensation of MARCM-based Clones in Drosophila melanogaster

Because of the structural and functional homology to the hair cells of the mammalian inner ear, the neurons that innervate the Drosophila external sense organs provide an excellent model system for the study of mechanosensation. This protocol describes a simple touch behavior in fruit flies which can be used to identify mutations that interfere with mechanosensation. The tactile stimulation of a macrochaete bristle on the thorax of flies elicits a grooming reflex from either the first or third leg. Mutations that interfere with mechanotransduction (such as NOMPC), or with other aspects of the reflex arc, can inhibit the grooming response. A traditional screen of adult behaviors would have missed mutants that have essential roles during development. Instead, this protocol combines the touch screen with mosaic analysis with a repressible cell marker (MARCM) to allow for only limited regions of homozygous mutant cells to be generated and marked by the expression of green fluorescent protein (GFP). By testing MARCM clones for abnormal behavioral responses, it is possible to screen a collection of lethal p-element mutations to search for new genes involved in mechanosensation that would have been missed by more traditional methods.

A Behavioral Assay for Mechanosensation of MARCM-based Clones in Drosophila melanogaster

In order to identify novel mutations affecting mechanosensation, we designed an assay that measures the behavioral response to tactile stimulation of fly bristles in mutant clones generated by the MARCM method. The combination of techniques allows for the identification of mechanosensitive mutations that would otherwise be lethal.

A Behavioral Essai pour mécanosensibilité de marcm-fondé clones dans<em&gt; Drosophila melanogaster</em

Because of the structural and functional homology to the hair cells of the mammalian inner ear, the neurons that innervate the Drosophila external sense ...

Measuring and Altering Mating Drive in Male Drosophila melanogaster

Despite decades of investigation, the neuronal and molecular bases of motivational states remain mysterious. We have recently developed a novel, reductionist, and scalable system for in-depth investigation of motivation using the mating drive of male Drosophila&#160;melanogaster (Drosophila), the methods for which we detail here. The behavioral paradigm centers on the finding that male mating drive decreases alongside fertility over the course of repeated copulations and recovers over ~3 d. In this system, the powerful neurogenetic tools available in the fly converge with the genetic accessibility and putative wiring diagram available for sexual behavior. This convergence allows rapid isolation and interrogation of small neuronal populations with specific motivational functions. Here we detail the design and execution of the satiety assay that is used to measure and alter courtship motivation in the male fly. Using this assay, we also demonstrate that low male mating drive can be overcome by stimulating dopaminergic neurons. The satiety assay is simple, affordable, and robust to influences of genetic background. We expect the satiety assay to generate many new insights into the neurobiology of motivational states.

Measuring and Altering Mating Drive in Male Drosophila melanogaster

This article describes a behavioral assay that uses male mating drive in Drosophila melanogaster to study motivation. Using this method, researchers can utilize advanced fly neurogenetic techniques to uncover the genetic, molecular, and cellular mechanisms that underlie this motivation.

Mesure et Altération Mating Drive à Male<em&gt; Drosophila melanogaster</em

Despite decades of investigation, the neuronal and molecular bases of motivational states remain mysterious. We have recently developed a novel, ...

A Temperature Gradient Assay to Determine Thermal Preferences of Drosophila Larvae

Many animals, including the fruit fly, Drosophila melanogaster, are capable of discriminating minute differences in&#160;environmental temperature, which enables them to seek out their preferred thermal landscape. To define the temperature preferences of larvae over a defined linear range, we developed an assay using a temperature gradient. To establish a single-directional gradient, two aluminum blocks are connected to independent water baths, each of which controls the temperature of individual blocks. The two blocks set the lower and upper limits of the gradient. The temperature gradient is established by placing an agarose-coated aluminum plate over the two water-controlled blocks so that the plate spans the distance between them. The ends of the aluminum plate that is set on the top of the water blocks defines the minimum and maximum temperatures, and the regions in-between the two blocks form a linear temperature gradient. The gradient assay can be applied to&#160;larvae of different ages and can be used to identify mutants that exhibit phenotypes, such as those with mutations affecting genes encoding TRP channels and opsins, which are required for temperature discrimination.

A Temperature Gradient Assay to Determine Thermal Preferences of Drosophila Larvae

Here, we present a protocol to determine the preferred environmental temperature of Drosophila larvae using a continuous thermal gradient.

Un dosage de Gradient de température pour déterminer les préférences thermiques de larves de drosophile

Many animals, including the fruit fly, Drosophila melanogaster, are capable of discriminating minute differences in&#160;environmental temperature, which ...

A Rapid Food-Preference Assay in Drosophila

To select food with nutritional value while avoiding the consumption of harmful agents, animals need a sophisticated and robust taste system to evaluate their food environment. The fruit fly, Drosophila melanogaster, is a genetically tractable model organism that is widely used to decipher the molecular, cellular, and neural underpinnings of food preference. To analyze fly food preference, a robust feeding method is needed. Described here is a two-choice feeding assay, which is rigorous, cost-saving, and fast. The assay is Petri-dish-based and involves the addition of two different foods supplemented with blue or red dye to the two halves of the dish. Then, ~70 prestarved, 2-4-day-old flies are placed in the dish and&#160;allowed to choose between blue and red foods in the dark for about 90 min. Examination of the abdomen of each fly is followed by the calculation of the preference index. In contrast to multiwell plates, each Petri dish takes only ~20 s to fill and saves time and effort. This feeding assay can be employed to quickly determine whether flies like or dislike a particular food.

A Rapid Food-Preference Assay in Drosophila

We present a protocol for a two-choice feeding assay for flies. This feeding assay is fast and easy to run and is suitable not only for small-scale laboratory research, but also for high-throughput behavioral screens in flies.

Un test rapide de préférence alimentaire à Drosophila

To select food with nutritional value while avoiding the consumption of harmful agents, animals need a sophisticated and robust taste system to evaluate ...

Egg Laying Assay: A Method to Quantify the Egg-Laying Behavior of C. elegans

Source: Lee, S. Y. and Kang, K. <a href="https://www.jove.com/video/60419/measuring-effects-bacteria-chemicals-on-intestinal-permeability">Measuring the Effect of Chemicals on the Growth and Reproduction of&#160;Caenorhabditis elegans</a>.&#160;J. Vis. Exp. (2017).
C. elegans egg-laying behavior can provide important information in developmental, reproductive, and anatomical studies.&#160; This video introduces the important steps of an egg laying assay and shows a sample experiment in which a drug is tested for reproductive toxicity.

Essai de ponte : une méthode pour quantifier le comportement de ponte de C. elegans

Source: Lee, S. Y. and Kang, K. Measuring the Effect of Chemicals on the Growth and Reproduction of&#160;Caenorhabditis elegans.&#160;J. Vis. Exp. 
...

JoVE Encyclopedia of Experiments

Biologie

Caenorhabditis elegans (worm)

Basic Methods

Island Assay: A High-Throughput Method to Evaluate Drosophila Locomotor Behavior

Source: Eidhof, I., et al. <a href="https://www.jove.com/video/55892/" target="_blank">High-throughput Analysis of Locomotor Behavior in the Drosophila Island Assay</a>. J. Vis. Exp. (2017).
This video describes the island assay, a behavioral method used to study Drosophila locomotion. The sample protocol features a setup for the assay compatible with semi-automated image analysis.

Island Assay : une méthode à haut débit pour évaluer le comportement locomoteur de la drosophile

Source: Eidhof, I., et al. High-throughput Analysis of Locomotor Behavior in the Drosophila Island Assay. J. Vis. Exp. (2017).
This video describes the ...

High Throughput Assay pour examiner les préférences de ponte de l'individu

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Island Assay : une méthode à haut débit pour évaluer le comportement locomoteur de la drosophile