Culture and Calcium Phosphate Transfection of Rat Hippocampal Primary Neurons
3:49
Whole-cell Recording with Light Activation
6:19
Results: PIRK Expression and Activation in Rat Hippocampal Neurons and Mouse Neocortex
7:26
Conclusion
Transcription
The overall goal of this procedure is to optically control a neuronal protein's function using a genetically encoded, unnatural amino acid in neurons. This method can help answer key questions about the contribution of a specific neuronal protein
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Here, a procedure to selectively activate a neuronal protein with a short pulse of light by genetically encoding a photo-reactive unnatural amino acid into a target neuronal protein expressed in neurons in culture or in vivo is presented.