Tissue Embedding, Thin Sectioning and Mounting for LAM
4:14
LAM
7:32
Results: Identification of Genes Expressed Only in the Apomictic Egg Cell
8:33
Conclusion
Transcription
The overall goal of this protocol for LAM is to isolate specific plant cells for the generation of high-integrity RNA for transcriptional analysis. The focus is on inaccessible cells, like egg cells, in sexual and apomictic boechera species. This
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Here we present a protocol for laser-assisted microdissection of specific plant cell types for transcriptional profiling. While the protocol is suitable for different species and cell types, the focus is on highly inaccessible cells of the female germline important for sexual and apomictic reproduction in the crucifer genus Boechera.