Preparing the Microchannel Device for Cell Seeding
2:37
Endothelial Cell Seeding and Long-term Perfusion
4:38
Immunofluorescent Staining and Imaging Calcium Concentration or Nitric Oxide Production
6:39
Data Analysis
7:35
Results: Endothelial Cell Culture Under Shear Stress
8:39
Conclusion
Transcription
The overall goal of this procedure is to develop a microfluidic device that allows endothelial cells to grow under physiologically relevant shear flow and to measure the agonist induced changes in their calcium and and nitric oxide production. Thi
Connectez-vous ou commencez votre essai gratuit pour accéder à ce contenu.
Primary human umbilical vein endothelial cells (HUVECs) were grown to confluence within a microfluidic network device. The endothelial cell junction and F-actin distributions were illustrated and the changes in intracellular calcium concentration and nitric oxide production in response to adenosine triphosphate (ATP) were quantified in real-time at individual cell levels.