Isolation of Leukemic Lymphocytes from Blood Samples of CCL Patients
2:09
Purification of Leukemic B Cells by Negative Isolation
3:27
Program the HPLC Method
5:07
Pretreatment with the Inhibitors and Incubation with the Substrate (AMP)
6:16
Samples Preparation for HPLC and HPLC Measurements of Purines
8:14
Results: Quantification of Purine Compounds in Freshly Purified CLL Cells from a Representative CD73+ Patient
9:36
Conclusion
Transcription
The overall goal of this procedure, is to use reverse phase high performance liquid chromotography to quantify purine metabolism in chronic lymphocytic leukemia or CLL cells measured in different culture conditions. This method has been used to de
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The protocol described here represents an easy and reproducible method that employs reverse phase high-performance liquid chromatography (RP-HPLC) to measure purine metabolism on chronic lymphocytic leukemia (CLL) cells cultured under different conditions.