Investigation of Bioconjugates by Gel Electrophoresis
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Results: Synthesis and Characterization of Cytochrome c Bioconjugates
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Conclusion
Transcription
The overall goal of synthesizing protein bioconjugates via cysteine-maleimide chemistry is to ensure specificity in the site of modification on the protein. Which in turn effects the activity of the resulting bioconjugate, for example, antibody dr
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This protocol details the important steps required for the bioconjugation of a cysteine containing protein to a maleimide, including reagent purification, reaction conditions, bioconjugate purification and bioconjugate characterization.