Fixation of Oocyte Tissue and Membrane Removal ("Rolling")
4:09
Antibody Staining of Drosophila Oocytes
6:47
Results: Visualization of Prophase and Metaphase in Drosophila Oocytes
8:15
Conclusion
Transcription
The overall goal of this procedure is to prepare Drosophila oocytes for the cytological examination of spindle assembly and chromosome orientation during meiosis I.This method can help answer key questions in the meiosis field, such as how spindle
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We present protocols for the collection, preparation, and imaging of mature Drosophila oocytes. These methods allow the visualization of chromosome behavior and spindle assembly and function during meiosis.