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Mouse Oocyte Microinjection, Maturation and Ploidy Assessment

29.5K Views

07:03 min

July 23rd, 2011

July 23rd, 2011

29,513 Views

0:05

Title

1:00

Mouse Oocyte Collection

2:28

Oocyte Microinjection and Maturation

4:14

Ploidy Analysis

6:04

A Euploid Metaphase II Egg

6:27

Conclusion

Oocytes are prone to aneuploidy due to errors in chromosome segregation during meiotic maturation. Aneuploid eggs can cause infertility, miscarriages or developmental disorders like Down syndrome. Here, we describe methods to introduce materials of choice into oocytes and methods to study meiotic maturation and assess ploidy.

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