Prepare Dilutions of the Assay-specific Reference Chemical and Water Extract
2:42
Prepare the Cell Suspension to Conduct the Fret Bioassay
4:05
Plate Cells in a 96-well Black Wall Clear-bottom Plate and Add Diluted Water Extract
4:54
Measure Cytotoxicity and Endocrine Activity Response
5:51
Results
7:22
Conclusion
Transcription
The overall goal of this standardized procedure is to screen water samples for endocrine active chemicals, using commercially-available, receptor-based cell assays. This method can help answer key questions in the water quality monitoring field, s
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A protocol to screen for endocrine activity in organic extracts of water samples, including treated wastewater effluent and surface (receiving) water, was adapted using commercially available division-arrested ("freeze and thaw") in vitro transactivation bioassays.