Results: Visualization of NF90-RBM3 Interactions in Nuclear and Cytoplasmic Fractions of Cells
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Conclusion
Transcription
The overall goal of this experiment is to compare the advantages and disadvantages of co-immunoprecipitation, or co-ip, and the proximity ligation assay, or pla, in visualizing protein protein interactions in different cellular compartments. The h
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Protein-protein interactions can occur in both the nucleus and the cytoplasm of a cell. To investigate these interactions, traditional co-immunoprecipitation and modern proximity ligation assay are applied. In this study, we compare these two methods to visualize the distribution of NF90-RBM3 interactions in the nucleus and the cytoplasm.