Imaging Drug-induced Ca2+ Transients and In Situ Kd Calibration
10:14
Results: In Situ Kd of CatchER+ in C2C12 Myoblast Cells
11:32
Conclusion
Transcription
The overall goal of this experiment is to display our protocols for the culture and transfection of HEK293 and C2C12 cells with CatchER plus and its application for monitoring ERSR receptor mediated calcium transients in the measurement of the NC2
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We present protocols for the application of our targeted genetically-encoded calcium indicator (GECI) CatchER+ for monitoring rapid calcium transients in the endoplasmic/sarcoplasmic reticulum (ER/SR) of HEK293 and skeletal muscle C2C12 cells using real-time fluorescence microscopy. A protocol for the in situ Kd measurement and calibration is also discussed.