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Monitoring ER/SR Calcium Release with the Targeted Ca2+ Sensor CatchER+

DOI :

10.3791/55822-v

May 19th, 2017

May 19th, 2017

14,490 Views

1Department of Chemistry, Center of Diagnostics and Therapeutics (CDT), Georgia State University

We present protocols for the application of our targeted genetically-encoded calcium indicator (GECI) CatchER+ for monitoring rapid calcium transients in the endoplasmic/sarcoplasmic reticulum (ER/SR) of HEK293 and skeletal muscle C2C12 cells using real-time fluorescence microscopy. A protocol for the in situ Kd measurement and calibration is also discussed.

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ER SR Calcium Release

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