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JoVE Journal

Neuroscience

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Stereological Estimation of Dopaminergic Neuron Number in the Mouse Substantia Nigra Using the Optical Fractionator and Standard Microscopy Equipment

17.6K Views

08:19 min

September 1st, 2017

September 1st, 2017

17,606 Views

0:05

Title

0:52

Tissue Processing

2:24

Acquisition of Images

4:20

Sequence of Stereological Assessment

6:37

Results: Comparison of Cell Counting Parameters

7:37

Conclusion

Transcription

The overall goal of this protocol is to describe the design-based stereological estimation of dopaminergic cell number in the mouse SN using standard microscopy equipment. This method is useful to estimate the number of dopaminergic neurons in the

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This work presents a step-by-step protocol for the unbiased stereological estimation of dopaminergic neuronal cell numbers in the mouse substantia nigra using standard microscopy equipment (i.e., a light microscope, a motorized object table (x, y, z plane), and public domain software for digital image analysis.

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